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Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium

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Abstract

Umbilical cord blood-derived marrow stromal cells (UCB-MSCs) with high proliferation capacity and immunomodulatory properties are considered to be a good candidate for cell-based therapies. But until now, little work has been focused on the differentiation of UCB-MSCs. In this work, UCB-MSCs were demonstrated to be negative for CD34 and CD45 expression but positive for CD90 and CD105 expression. The gate values of UCB-MSCs for CD90 and CD105 were 99.3 and 98.6 %, respectively. Two weeks after treatment, the percentage of neuron-like cells differentiated from UCB-MSCs was increased to 84 ± 12 % in the experimental group [treated with olfactory ensheathing cells (OECs)-conditioned medium] and they were neuron-specific enolase positive; few neuron-like cells were found in the control group (without OECs-conditioned medium). Using whole-cell recording, sodium and potassium currents were recorded in UCB-MSCs after differentiation by OECs. Thus, human UCB-MSCs could be differentiated to neural cells by secreted secretion from OECs and exhibited electrophysiological properties similar to mature neurons after 2 weeks post-induction. These results imply that OECs can be used as a new strategy for stem cell differentiation and provide an alternative neurogenesis pathway for generating sufficient numbers of neural cells for cell therapy.

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Authors and Affiliations

  1. Department of Neurosurgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan Province, 410008, China

    Yu Zeng, Yunsheng Liu, Jingfang Liu, Zhenyan Li, Xin Chen, Kui Yang, Chuntao Li & Zhixiong Liu

  2. Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China

    Mingqiang Rong

  3. Department of Neurosurgery, Second Affiliated Hospital of Hunan Normal University, 163 Hospital of PLA, Changsha, Hunan Province, 410008, China

    Ming Lu & Xiaoyu Tao

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  1. Yu Zeng
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  2. Mingqiang Rong
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  3. Yunsheng Liu
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  4. Jingfang Liu
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  5. Ming Lu
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  8. Xin Chen
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  10. Chuntao Li
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  11. Zhixiong Liu
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Correspondence to Ming Lu or Zhixiong Liu.

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Zeng, Y., Rong, M., Liu, Y. et al. Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium. Neurochem Res 38, 2483–2489 (2013). https://doi.org/10.1007/s11064-013-1186-x

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  • DOI: https://doi.org/10.1007/s11064-013-1186-x

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