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The Role of HSPA12B in Regulating Neuronal Apoptosis

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Abstract

Heat shock protein A12B (HSPA12B) is the newest member of a recently defined subfamily of proteins distantly related to the 70-kDa family of heat shock proteins (HSP70) family. HSP70s play a crucial role in protecting cells, tissues, organs and animals from various noxious conditions. Here we studied the dynamic expression changes and localization of HSPA12B after middle cerebral artery occlusion (MCAO) with reperfusion induced ischemic insult processes in adult rats. Apoptosis, as indicated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, was also increased in the peri-ischemic cortex compared to non-ischemic hemisphere. The expression of HSPA12B was strongly induced in the ischemic hemisphere of MCAO reperfusion rats in vivo. In vitro studies indicated that the up-regulation of HSPA12B may be involved in oxygen-glucose deprivation-induced PC12 cell death. And knockdown of HSPA12B in cultured differentiated PC12 cells by siRNA showed that HSPA12B inhibited the expression of active caspase-3. Collectively, these results suggested that HSPA12B may be required for protecting neurons from ischemic insults.

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Abbreviations

HSPA12B:

Heat shock protein A12B

MCAO:

Middle cerebral artery occlusion

OGD:

Oxygen glucose deprivation

CNS:

Central nervous system

NGF:

Nerve growth factor

NSS:

Neurological severity scores

PAGE:

Polyacrylamide gel electrophoresis

ECL:

Enhanced chemiluminescence system

RT-PCR:

Reverse transcriptase PCR

MAP2:

Microtubule-associated protein 2

TUNEL:

Terminal deoxynucleotidyl transferase dUTP nick end labeling

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31070723 and No. 81070922); A project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Lihua Kang, Guowei Zhang, Jing Li, Lin Zhu & Qiyun Wu

  2. Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, 226001, People’s Republic of China

    Lihua Kang & Guowei Zhang

  3. Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, People’s Republic of China

    Yaohua Yan, Kaifu Ke, Xinmin Wu & Yilu Gao

  4. Department of Osteology, Affiliated Jiangyin Hospital, Nantong University, Wuxi, 214400, People’s Republic of China

    Lihua Kang, Guowei Zhang, Lin Zhu & Zhengming Zhou

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  1. Lihua Kang
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  2. Guowei Zhang
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Corresponding authors

Correspondence to Qiyun Wu or Zhengming Zhou.

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Lihua Kang and Guowei Zhang contributed equally to this work.

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Kang, L., Zhang, G., Yan, Y. et al. The Role of HSPA12B in Regulating Neuronal Apoptosis. Neurochem Res 38, 311–320 (2013). https://doi.org/10.1007/s11064-012-0922-y

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  • DOI: https://doi.org/10.1007/s11064-012-0922-y

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