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Alterations in the Expression of the Anti-Apoptotic Factor HAX-1 upon Seizures-Induced Hippocampal Injury in the Neonatal Rat Brain

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Abstract

HS1-associated protein X1 (HAX-1) is a mitochondrial protein which interacts with a diverse group of molecules such as inflammatory cytokines; interleukin-1, hematopoietic lineage specific protein-1 and vimentin. It has been reported that HAX-1 may act as antiapoptotic protein in HeLa- and Jurkat cells after Fas-treatment, irradiation or serum deprivation. This underlines the evidence that HAX-1 might be involved in both receptor- and mitochondria-mediated apoptosis pathways. However, the role of HAX-1 in neuronal death induced by status epilepticus in the immature brain has not been reported. In this study, we performed a status epilepticus in rats and investigated the dynamic changes of HAX-1 expression, HtrA2 distribution and caspase-3 activation in the hippocampus. Western blot and immunohistochemistry analysis revealed that HAX-1 was expressed at very low levels in the hippocampus. Status epilepticus in the immature brain significantly induced increased cytosolic accumulation of HAX-1 in a biphasic manner, induced an upregulation of HtrA2 and enhanced caspase-3 activity in the selectively vulnerable hippocampal CA1-subfield. Taken together, these results suggested that HAX-1 is probably involved in the pathophysiology of cell death induced by epilepsy.

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Acknowledgments

We thank Prof. Jörg Stehle for continuous support to our work. This study was funded in part by research grant from the Adolf-Messer-Stiftung (Grant to Dr. A. Rami).

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Authors and Affiliations

  1. Institut für Zelluläre und Molekulare Anatomie (Anatomie III), Klinikum der Johann Wolfgang von Goethe-Universität, Theodor-Stern-Kai 7, 60590, Franfurt/Main, Germany

    A. Rami, M. Kim & A. Langhagen

  2. Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA

    J. Niquet

  3. Dr. Senckenbergische Anatomie, Anatomie III, Universitätsklinikum, Theodor-Stern-Kai 7, 60590, Frankfurt/Main, Germany

    A. Rami

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  1. A. Rami
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  2. M. Kim
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  4. A. Langhagen
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Correspondence to A. Rami.

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Rami, A., Kim, M., Niquet, J. et al. Alterations in the Expression of the Anti-Apoptotic Factor HAX-1 upon Seizures-Induced Hippocampal Injury in the Neonatal Rat Brain. Neurochem Res 37, 116–125 (2012). https://doi.org/10.1007/s11064-011-0589-9

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  • DOI: https://doi.org/10.1007/s11064-011-0589-9

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