ORIGINAL PAPER

Neurochemical Research

, Volume 35, Issue 12, pp 2144-2153

Modulation of PARP-1 and PARP-2 Expression by L-carnosine and Trehalose After LPS and INFγ-Induced Oxidative Stress

  • Vittoria Spina-PurrelloAffiliated withDepartment of Chemical Sciences, Section of Biochemistry and Molecular Biology, Medical Faculty, University of Catania Email author 
  • , Salvatrice GilibertoAffiliated withDepartment of Chemical Sciences, Section of Biochemistry and Molecular Biology, Medical Faculty, University of Catania
  • , Vincenza BarresiAffiliated withDepartment of Chemical Sciences, Section of Biochemistry and Molecular Biology, Medical Faculty, University of Catania
  • , Vincenzo G. NicolettiAffiliated withDepartment of Chemical Sciences, Section of Biochemistry and Molecular Biology, Medical Faculty, University of Catania
  • , Anna Maria Giuffrida StellaAffiliated withDepartment of Chemical Sciences, Section of Biochemistry and Molecular Biology, Medical Faculty, University of Catania
  • , Enrico RizzarelliAffiliated withDepartment of Chemical Sciences, University of CataniaIstituto Biostrutture e Bioimmagini CNR Sez. di Catania

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Abstract

Poly(ADP-ribose) polymerases (PARPs) play a crucial role in DNA damage surveillance through their nick sensor functions. Since PARPs’ over activation leads to an excessive consumption of NAD+ and ATP depletion, these enzymes also are involved in the early events of programmed cell death as well as in necrosis. In order to verify the protective action of L-carnosine and trehalose against NO induced cell death, in the present study we examined their effects on the expression of PARP-1, PARP-2 and iNOS in primary rat astrocyte and oligodendrocyte cells, treated with lipopolysaccharide (LPS) and interferon gamma (INFγ), through semi-quantitative PCR and western analysis. To further characterize the molecular mechanisms underlying L-carnosine and trehalose action, we measured cell viability, nitrite production and LDH release. The data obtained clearly demonstrate that in the stress model employed L-carnosine and trehalose down regulate PARP-1 and PARP-2 expression in both cell phenotypes, thus suggesting their possible application in clinical trials.

Keywords

Astrocytes Oligodendrocytes PARP iNOS L-Carnosine Trehalose