Original Paper

Neurochemical Research

, Volume 35, Issue 1, pp 152-161

Combination Treatment with Resveratrol and Sulforaphane Induces Apoptosis in Human U251 Glioma Cells

  • Hao JiangAffiliated withDepartment of Neurology, Henry Ford Hospital Email author 
  • , Xia ShangAffiliated withDepartment of Neurology, Henry Ford Hospital
  • , Hongtao WuAffiliated withDepartment of Neurosurgery, Henry Ford Hospital
  • , Grace HuangAffiliated withDepartment of Neurology, Henry Ford Hospital
  • , Yiyang WangAffiliated withDepartment of Neurology, Henry Ford Hospital
  • , Shaza Al-HolouAffiliated withDepartment of Neurology, Henry Ford Hospital
  • , Subhash C. GautamAffiliated withDepartment of Surgery, Henry Ford Hospital
  • , Michael ChoppAffiliated withDepartment of Neurology, Henry Ford HospitalDepartment of Physics, Oakland University

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Abstract

Resveratrol is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Sulforaphane belongs to the family of isothiocyanates and is highly enriched in cruciferous vegetables. Our previous study showed that resveratrol, when used at high concentrations, inhibited cell proliferation, caused the cell cycle arrest and induced apoptotic cell death in glioma cells. In the current study, we tested the effect of combination treatment with resveratrol and sulforaphane, when both were used at low concentrations, on cell proliferation, migration and death in human U251 glioma cells. Our study shows that combination treatment with resveratrol and sulforaphane inhibits cell proliferation and migration, reduces cell viability, induces lactate dehydrogenase release, decreases pro-survival Akt phosphorylation and increases caspase-3 activation. The use of combination of bioactive food components, such as resveratrol and sulforaphane, may be a viable approach for the treatment of glioma.

Keywords

Resveratrol Sulforaphane Glioma Apoptosis Caspase-3 Akt