Neurochemical Research

, 35:152

Combination Treatment with Resveratrol and Sulforaphane Induces Apoptosis in Human U251 Glioma Cells

  • Hao Jiang
  • Xia Shang
  • Hongtao Wu
  • Grace Huang
  • Yiyang Wang
  • Shaza Al-Holou
  • Subhash C. Gautam
  • Michael Chopp
Original Paper

DOI: 10.1007/s11064-009-0040-7

Cite this article as:
Jiang, H., Shang, X., Wu, H. et al. Neurochem Res (2010) 35: 152. doi:10.1007/s11064-009-0040-7

Abstract

Resveratrol is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Sulforaphane belongs to the family of isothiocyanates and is highly enriched in cruciferous vegetables. Our previous study showed that resveratrol, when used at high concentrations, inhibited cell proliferation, caused the cell cycle arrest and induced apoptotic cell death in glioma cells. In the current study, we tested the effect of combination treatment with resveratrol and sulforaphane, when both were used at low concentrations, on cell proliferation, migration and death in human U251 glioma cells. Our study shows that combination treatment with resveratrol and sulforaphane inhibits cell proliferation and migration, reduces cell viability, induces lactate dehydrogenase release, decreases pro-survival Akt phosphorylation and increases caspase-3 activation. The use of combination of bioactive food components, such as resveratrol and sulforaphane, may be a viable approach for the treatment of glioma.

Keywords

ResveratrolSulforaphaneGliomaApoptosisCaspase-3Akt

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hao Jiang
    • 1
  • Xia Shang
    • 1
  • Hongtao Wu
    • 2
  • Grace Huang
    • 1
  • Yiyang Wang
    • 1
  • Shaza Al-Holou
    • 1
  • Subhash C. Gautam
    • 3
  • Michael Chopp
    • 1
    • 4
  1. 1.Department of NeurologyHenry Ford HospitalDetroitUSA
  2. 2.Department of NeurosurgeryHenry Ford HospitalDetroitUSA
  3. 3.Department of SurgeryHenry Ford HospitalDetroitUSA
  4. 4.Department of PhysicsOakland UniversityRochester HillsUSA