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Physicochemical characterization of 3,6-diHydroxyflavone binding BSA immobilized on PEG-coated silver nanoparticles

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Abstract

Studies based on silver nanoparticles (SNPs) and polyethylene glycols (PEGs) are mainly in the pharmaceutical field, with PEG as good “vehicle” to transport protein-based drugs. In this work, physicochemical characteristics of 3,6-diHydroxyflavone (3,6-diHF) binding bovine serum albumin (BSA) on PEG (Tween20, L64, and Myrj52)-coated SNPs have been investigated by steady-state and time-resolved fluorescence spectroscopy. These interactions give rise to the formation of intermolecular and intramolecular H bonds. As a subject of interest, the effect of temperature (30–60 °C) on the H bonds was studied by steady-state fluorescence. The size distribution and zeta potential of SNPs were determined by dynamic light scattering (DLS). Scanning electron microscopy (SEM) analysis revealed the spherical nature of particles with average diameter ~40–80 nm. The structure, stability, dynamics, and conformational changes in adsorbed BSA protein on the PEG-coated SNPs surface have been also investigated by steady-state/lifetime fluorescence and circular dichroism spectroscopy. The results have relevance in the oxidative stress and drug delivery processes.

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Acknowledgements

This work was supported by a grant of the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, project number PN-II-RU-TE-2012-3-0055.

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Authors and Affiliations

  1. Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, Splaiul Independentei 202, 060021, Bucharest, Romania

    Mariana Voicescu & Jose M. Calderon-Moreno

  2. Department of Physical Chemistry, University of Bucharest, Bd. Regina Elisabeta 4-12, 030018, Bucharest, Romania

    Sorana Ionescu

  3. Polymer Department, National R&D Institute for Chemistry and Petrochemistry ICECHIM, Splaiul Independentei 202, 060021, Bucharest, Romania

    Cristina L. Nistor

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  1. Mariana Voicescu
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  2. Sorana Ionescu
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  3. Jose M. Calderon-Moreno
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  4. Cristina L. Nistor
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Correspondence to Mariana Voicescu.

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Voicescu, M., Ionescu, S., Calderon-Moreno, J.M. et al. Physicochemical characterization of 3,6-diHydroxyflavone binding BSA immobilized on PEG-coated silver nanoparticles. J Nanopart Res 19, 38 (2017). https://doi.org/10.1007/s11051-016-3727-0

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