Abstract
An in-depth study of a novel functionalization of carbon nanotubes for their application as protein and DNA carriers is presented. First, the optimum conditions for the dispersion of single-walled carbon nanotubes (SWCNTs) with amphiphilic polypeptides were obtained, and the SWCNT–polypeptide complexes were characterized by different techniques (UV–Vis-NIR, CD, and AFM). Based on the properties of the SWCNT–polypeptide complexes, a model that characterizes the adsorption of natural proteins onto SWCNT was described for the first time. This model predicts the adsorption of natural proteins on SWCNTs based on the protein structure and composition, and therefore, allows the design of methods for the preparation of SWCNT–protein complexes. Besides, the use of cationic-designed amphiphilic polypeptides to disperse SWCNTs is applied for subsequent and efficient binding of DNA to carbon nanotubes by a bilayer approach. Therefore, in this article, we develop procedures for the use of SWCNTs as protein and DNA carriers. The systems were delivered into cells showing that the efficiency of delivery is affected by the charge of the complexes, which has important implications in the use of SWCNT as platforms for protein and DNA binding and subsequent use as delivery systems.
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References
Besteman K, Lee JO, Wiertz FGM, Heering HA, Dekker C (2003) Enzyme-coated carbon nanotubes as single-molecule biosensors. Nano Lett 3:727–730
Bianco A, Kostarelos K, Prato M (2005) Applications of carbon nanotubes in drug delivery. Curr Opin Chem Biol 9:674–679
Britz DA, Khlobystov AN (2006) Noncovalent interactions of molecules with single walled carbon nanotubes. Chem Soc Rev 35:637–659
Chen RJ, Zhang Y, Wang D, Dai H (2001) Non-covalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization. J Am Chem Soc 123:3838–3839
Chen RJ, Bangsaruntip S, Drouvalakis KA, Kam NWS, Shim M, Li Y et al (2003) Non-covalent functionalization of carbon nanotubes for highly specific electronic biosensors. PNAS 100:4984–4989
Dukovic G, Balaz M, Doak P, Berova ND, Zheng M, Mclean RS et al (2006) Racemic single-walled carbon nanotubes exhibit circular dichroism when wrapped with DNA. J Am Chem Soc 128:9004–9005
Enyashin AN, Gemming S, Seifert G (2007) DNA-wrapped carbon nanotubes. Nanotechnology 18:245702
Heister E, Neves V, Tîlmaciu C, Lipert K, Sanz Beltrán V, Coley HM et al (2009) Triple functionalisation of single-walled carbon nanotubes with doxorubicin, a monoclonal antibody, and a fluorescent marker for targeted cancer therapy. Carbon 47:2152–2160
Heller DA, Jeng ES, Yeung TK, Martinez BM, Moll AE, Gastala JB et al (2006) Optical detection of DNA conformational polymorphism on single walled carbon nanotubes. Science 311:508–511
Huang W, Taylor S, Fu K, Lin Y, Zhang D, Hanks TW et al (2002) Attaching proteins to carbon nanotubes via diimide-activated amidation. Nano Lett 2:311–314
Jeynes JCG, Mendoza E, Chow DCS, Watts PCP, McFadden J, Silva SRP (2006) Generation of chemically unmodified pure single-walled carbon nanotubes by solubilizing with RNA and treatment with ribonuclease A. Adv Mater 18:1598–1602
Ju SY, Kopcha WP, Papadimitrakopoulos F (2009) Brightly fluorescent single-walled carbon nanotubes via an oxygen-excluding surfactant organization. Science 323:1319–1323
Karajanagi SS, Yang H, Asuri P, Sellito E, Dordick JS, Kane RS (2006) Protein-assisted solubilization of single-walled carbon nanotubes. Langmuir 22:1392–1395
Kim SN, Rusling JF, Papadimitrakopoulos F (2007) Carbon nanotubes for electronic and electrochemical detection of biomolecules. Adv Mater 19:3214–3228
Kurppa K, Jiang H, Szilvay GR, Nasibulin AG, Kauppinen EI, Linder MB (2007) Controlled hybrid nanostructures through protein-mediated non-covalent functionalization of carbon nanotubes. Angew Chem Int Ed Engl 46:6446–6449
Liu Z, Tabakman S, Welsher K, Hongjie Dai H (2009) Carbon nanotubes in biology and medicine: in vitro and in vivo detection, imaging and drug delivery. Nano Res 2:85–120
Matsuura K, Saito T, Okazaki T, Ohshima S, Yumura M, Iijima S (2006) Selectivity of water-soluble proteins in single-walled carbon nanotube dispersions. Chem Phys Lett 429:497–502
Pantarotto D, Partidos CD, Graff R, Hoebeke J, Briand JP, Prato M et al (2003) Synthesis, structural characterization, and immunological properties of carbon nanotubes functionalized with peptides. J Am Chem Soc 125:6160–6164
Peng X, Komatsu N, Bhattacharya S, Shimawaki T, Aonuma S, Kimura T et al (2007) Optically active single-walled carbon nanotubes. Nat Nanotechnol 2:361–365
Salvador-Morales C, Flahaut E, Sim E, Sloan J, Green MLH, Sim RB (2006) Complement activation and protein adsorption by carbon nanotubes. Mol Immunol 43:193–201
Salzmann CG, Ward MAH, Jacobs RMJ, Tobias G, Green MLH (2007) Interaction of tyrosine-, tryptophan-, and lysine-containing polypeptides with single-wall carbon nanotubes and its relevance for the rational design of dispersing agents. J Phys Chem C 111:18520–18524
Salzmann CG, Lee GKC, Ward MAH, Chu BTT, Green MLH (2008) Highly hydrophilic and stable polypeptide/single-wall carbon nanotube conjugates. J Mater Chem 18:1977–1983
Shim M, Kam NWS, Chen RJ, Li Y, Dai H (2002) Functionalization of carbon nanotubes for biocompatibility and biomolecular recognition. Nano Lett 2:285–288
Singh R, Pantarotto D, McCarthy D, Chaloin O, Hoebeke JN, Partidos CD et al (2005) Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors. J Am Chem Soc 127:4388–4396
Ziegler KJ (2005) Developing implantable optical biosensors. Trends Biotechnol 23(9):440–444
Zorbas V, Ortiz-Acevedo A, Dalton AB, Yoshida MM, Dieckmann GR, Draper RK, Baughman RH, Yacaman MJ, Musselman IH (2004) Preparation and characterization of individual peptide-wrapped single-walled carbon nanotubes. J Am Chem Soc 126:7222–7227
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This study has been performed within the framework of the FP6Marie Curie Research Training Network “CARBIO” (RTN-CT-2006-035616) funded by the European Union. The authors also acknowledge funding received from the EPSRC Portfolio Partnership award.
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Sanz, V., Coley, H.M., Silva, S.R.P. et al. Modeling the binding of peptides on carbon nanotubes and their use as protein and DNA carriers. J Nanopart Res 14, 695 (2012). https://doi.org/10.1007/s11051-011-0695-2
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DOI: https://doi.org/10.1007/s11051-011-0695-2