Journal of Nanoparticle Research

, 13:2675

Interlaboratory comparison of size and surface charge measurements on nanoparticles prior to biological impact assessment

  • G. Roebben
  • S. Ramirez-Garcia
  • V. A. Hackley
  • M. Roesslein
  • F. Klaessig
  • V. Kestens
  • I. Lynch
  • C. M. Garner
  • A. Rawle
  • A. Elder
  • V. L. Colvin
  • W. Kreyling
  • H. F. Krug
  • Z. A. Lewicka
  • S. McNeil
  • A. Nel
  • A. Patri
  • P. Wick
  • M. Wiesner
  • T. Xia
  • G. Oberdörster
  • K. A. Dawson
Perspectives

DOI: 10.1007/s11051-011-0423-y

Cite this article as:
Roebben, G., Ramirez-Garcia, S., Hackley, V.A. et al. J Nanopart Res (2011) 13: 2675. doi:10.1007/s11051-011-0423-y

Abstract

The International Alliance for NanoEHS Harmonization (IANH) organises interlaboratory comparisons of methods used to study the potential biological impacts of nanomaterials. The aim of IANH is to identify and reduce or remove sources of variability and irreproducibility in existing protocols. Here, we present results of the first IANH round robin studies into methods to assess the size and surface charge of suspended nanoparticles. The test materials used (suspensions of gold, silica, polystyrene, and ceria nanoparticles, with [primary] particles sizes between 10 nm and 80 nm) were first analysed in repeatability conditions to assess the possible contribution of between-sample heterogeneity to the between-laboratory variability. Reproducibility of the selected methods was investigated in an interlaboratory comparison between ten different laboratories in the USA and Europe. Robust statistical analysis was used to evaluate within- and between-laboratory variability. It is shown that, if detailed shipping, measurement, and reporting protocols are followed, measurement of the hydrodynamic particle diameter of nanoparticles in predispersed monomodal suspensions using the dynamic light scattering method is reproducible. On the other hand, measurements of more polydisperse suspensions of nanoparticle aggregates or agglomerates were not reproducible between laboratories. Ultrasonication, which is commonly used to prepare dispersions before cell exposures, was observed to further increase variability. The variability of the zeta potential values, which were also measured, indicates the need to define better surface charge test protocols and to identify sources of variability.

Keywords

NanoparticleParticle surface chargeInterlaboratory comparisonReproducibilityPolydispersityToxicologyHealth and safety implications

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • G. Roebben
    • 1
  • S. Ramirez-Garcia
    • 2
  • V. A. Hackley
    • 3
  • M. Roesslein
    • 4
  • F. Klaessig
    • 5
  • V. Kestens
    • 1
  • I. Lynch
    • 2
  • C. M. Garner
    • 6
  • A. Rawle
    • 7
  • A. Elder
    • 8
  • V. L. Colvin
    • 9
  • W. Kreyling
    • 10
  • H. F. Krug
    • 4
  • Z. A. Lewicka
    • 9
  • S. McNeil
    • 11
  • A. Nel
    • 12
  • A. Patri
    • 11
  • P. Wick
    • 4
  • M. Wiesner
    • 13
  • T. Xia
    • 12
  • G. Oberdörster
    • 8
  • K. A. Dawson
    • 2
  1. 1.Institute for Reference Materials and MeasurementsJoint Research Centre of the European CommissionGeelBelgium
  2. 2.Centre for BioNano InteractionsUniversity College DublinBelfieldIreland
  3. 3.Material Measurement LaboratoryNational Institute of Standards & TechnologyGaithersburgUSA
  4. 4.EMPAGallenSwitzerland
  5. 5.Pennsylvania Bio Nano Systems LLCDoylestownUSA
  6. 6.Garner Nanotechnology SolutionsPleasantonUSA
  7. 7.Malvern Instruments IncWestboroughUSA
  8. 8.Department of Environmental MedicineUniversity of RochesterRochesterUSA
  9. 9.Department of ChemistryRice UniversityHoustonUSA
  10. 10.Helmholtz Zentrum MuenchenInstitute of Lung Biology and DiseaseNeuherberg/MunichGermany
  11. 11.Nanotechnology Characterization Laboratory, Advanced Technology ProgramSAIC-Frederick, IncFrederickUSA
  12. 12.Division of Nano MedicineDepartment of Medicine at UCLALos AngelesUSA
  13. 13.Department of Civil and Environmental EngineeringDuke UniversityDurhamUSA