Abstract
In this article, water-soluble CdTe/CdS quantum dots (QDs) were synthesized in aqueous solution with captosuccinic acid as stabilizer. The absorption and fluorescence spectra showed that the as-prepared QDs had good optical properties. It was observed that the quantum yield (QY) of QDs was greatly increased after a heating–cooling cycle (from 22 to 41%). Then, the QDs were used to prepare fluorescent probes. The experiment results showed that the transferrin (Tf) could conjugate to QDs effectively and the HepG2 cells could be recognized successfully. This study is of great significance for the preparation of high-quality QDs and their applications in life science.
Similar content being viewed by others
References
Chan WCW, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–2018
Gao M, Kirstein S, Rogach AL (1998) Strongly photoluminescent CdTe nanocrystals by proper surface modification. J Phys Chem B 102:8360–8363
Gaponik N, Talapin DV, Rogach AL, Hoppe K, Shevchenko EV, Kornowski A, Eychmuller A, Weller H (2002) Thiol-capping of CdTe nanocrystals: an alternative to organometallic synthetic routes. J Phys Chem B 106:7177–7185
Gardner HC, Gallardo DE, Bertoni C, Dunn S (2006) Temperature shifted photoluminescence in CdTe nanocrystals. Proc SPIE 6195:61950N1–61950N8
Hoshino A, Hanaki K, Suzuki K, Yamamoto K (2004) Applications of T-lymphoma labeled with fluorescent quantum dots to cell tracing markers in mouse body. Biochem Biophys Res Commun 314:46–53
Hua XF, Liu TC, Cao YC, Liu B, Wang HQ, Wang JH, Huang ZL, Zhao YD (2006) Characterization of the coupling of quantum dots and immunoglobulin antibodies. Anal Bioanal Chem 386:1665–1671
Huang ZL, Lei H, Li N, Qiu ZR, Wang HZ, Guo JD, Luo Y, Zhong ZP, Liu XF, Zhou ZH (2003) Novel heterocycle-based organic molecules with two-photon induced blue fluorescent emission. J Mater Chem 13:708–711
Jamieson T, Bakhshi R, Petrova D, Pocock R, Imani M, Seifalian AM (2007) Biological applications of quantum dots. Biomaterials 28:4717–4732
Koch AM, Reynolds F, Kircher MF, Merkle HP, Weissleder R, Josephson L (2003) Uptake and metabolism of a dual fluorochrome tat-nanoparticle in HeLa cells. Bioconjugate Chem 14:1115–1121
Liu TC, Huang ZL, Wang HQ, Wang JH, Li XQ, Zhao YD, Luo QM (2006) Temperature-dependent photoluminescence of water-soluble quantum dots for a bioprobe. Anal Chim Acta 559:120–123
Mamedova NN, Kotov NA, Rogach AL, Studer J (2001) Albumin-CdTe nanoparticles bioconjugates: preparation, structure, and interunit energy transfer with antenna effect. Nano Lett 1:281–286
Marcel BJ, Mario M, Peter G, Shimon W, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013–2016
Peng H, Zhang L, Soeller C, Travas-Sejdic J (2007) Preparation of water-soluble CdTe/CdS core/shell quantum dots with enhanced photostability. J Lumin 127:721–726
Rajh T, Mićić OI, Nozik AJ (1993) Synthesis and characterization of surface-modified colloidal cadmium telluride quantum dots. J Phys Chem 97:11999–12003
Su YY, He Y, Lu HT, Sai LM, Li QN, Li WX, Wang LH, Shen PP, Huang Q, Fan CH (2009) The cytotoxicity of cadmium based, aqueous phase-synthesized, quantum dots and its modulation by surface coating. Biomaterials 30:19–25
Talapin DV, Rogach AL, Shevchenko EV, Kornowski A, Haase M, Weller H (2002) Dynamic distribution of growth rates within the ensembles of colloidal II–VI and III–V semiconductor nanocrystals as a factor governing their photoluminescence efficiency. J Am Chem Soc 124:5782–5790
Tekle C, Deurs BV, Sandvig K, Iversen TG (2008) Cellular trafficking of quantum dot-ligand bioconjugates and their induction of changes in normal routing of unconjugated ligands. Nano Lett 8:1858–1865
Wang SP, Mamedova N, Kotov NA, Chen W, Studer J (2002) Antigen/antibody immunocomplex from CdTe nanoparticle bioconjugates. Nano Lett 2:817–822
Wang HQ, Liu TC, Cao YC, Huang ZL, Wang JH, Li XQ, Zhao YD (2006a) A flow cytometric assay technology based on quantum dots-encoded beads. Anal Chim Acta 580:18–23
Wang Q, Kuo Y, Wang Y, Shin G, Ruengruglikit C, Huang Q (2006b) Luminescent properties of water-Soluble denatured bovine serum albumin-coated CdTe quantum dots. J Phys Chem B 110:16860–16866
Wang CL, Zhang H, Zhang JH, Li MJ, Sun HZ, Yang B (2007a) Application of ultrasonic irradiation in aqueous synthesis of highly fluorescent CdTe/CdS core-shell nanocrystals. J Phys Chem C 111:2465–2469
Wang JH, Wang HQ, Zhang HL, Li XQ, Hua XF, Cao YC, Huang ZL, Zhao YD (2007b) Purification of denatured bovine serum albumin coated CdTe quantum dots for sensitive detection of silver(I) ions. Anal Bioanal Chem 388:969–974
Wang JH, Wang HQ, Li YQ, Zhang HL, Li XQ, Hua XF, Cao YC, Huang ZL, Zhao YD (2008) Modification of CdTe quantum dots as temperature-insensitive bioprobes. Talanta 74:724–729
Wuister SF, Donegá CDM, Meijerink A (2004) Luminescence temperature antiquenching of water-Soluble CdTe quantum dots: role of the solvent. J Am Chem Soc 126:10397–10402
Yang MM, Yang P, Zhang LW (1994) Study on the interaction of protein and caffeic acid by fluorescence method. Chin Sci Bull 39:31–35
Yang WH, Li WW, Dou HJ, Sun K (2008) Hydrothermal synthesis for high-quality CdTe quantum dots capped by cysteamine. Mater Lett 62:2564–2566
Yu Y, Lai Y, Zheng X, Wu J, Long Z, Liang C (2007) Synthesis of functionalized CdTe/CdS QDs for spectrofluorimetric detection of BSA. Spectrochim Acta A 68:1356–1361
Zhang H, Zhou Z, Yang B, Gao MY (2003) The influence of carboxyl groups on the photoluminescence of mercaptocarboxylic acid-stabilized CdTe nanoparticles. J Phys Chem B 107:8–13
Zheng Y, Gao S, Ying JY (2007) Glutathione-capped CdTe QDs: synthesis and applications in cell imaging. Adv Mater 19:376–380
Acknowledgments
This study was supported by the National Natural Science Foundation of China (Grant No. 30670553), National High Technology Research and Development Program of China (863 Program: 2007AA10Z328). We also thank Analytical and Testing Center (HUST) for the help of measurement. This study was also supported by the Graduate Innovation Fund of Huazhong University of Science and Technology (HF05222007170).
Author information
Authors and Affiliations
Corresponding author
Additional information
Jian-Hao Wang and Hai-Li Zhang contributed equally to this study.
Rights and permissions
About this article
Cite this article
Wang, JH., Zhang, HL., Li, YQ. et al. A feasible method of improving the quantum yield of CdTe/CdS quantum dots by the first heating–cooling cycle and their application in cancer cell recognition. J Nanopart Res 12, 1687–1695 (2010). https://doi.org/10.1007/s11051-010-9866-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11051-010-9866-9