Abstract
We report a simple and novel method for surface biofunctionalization onto recently reported Ni80Fe20 permalloy nanoparticles (~71 nm) and the immobilization of a model protein, IgG from human serum. The strategy of protein immobilization involved attachment of histidine-tagged streptavidin to the Ni80Fe20 nanoparticles via a non-covalent ligand binding followed by biotinylated human IgG binding on the nanoparticle surface using the specific high affinity avidin–biotin interaction. The biofunctionalization of Ni80Fe20 permalloy nanoparticles was confirmed by Fourier Transform InfraRed (FTIR) spectroscopy and protein denaturing gel electrophoresis (lithium dodecyl sulfate-polyacrylamide gel electrophoresis, LDS-PAGE). This protocol for surface functionalization of the novel nanometer-sized Ni80Fe20 permalloy particles with biological molecules could open diverse applications in disease diagnostics and drug delivery.
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Acknowledgments
This work was financially supported by the Innovation Projects Fund National and International Research Alliances Program of Queensland Government, Australia. This work was in part supported by Key Project of Science and Technology, the Ministry of Education of China (No. 108039).
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Qin, G.W., Darain, F., Wang, H. et al. Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications. J Nanopart Res 13, 45–51 (2011). https://doi.org/10.1007/s11051-010-0101-5
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DOI: https://doi.org/10.1007/s11051-010-0101-5