Brief communication

Journal of Nanoparticle Research

, Volume 13, Issue 1, pp 45-51

Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications

  • Gaowu W. QinAffiliated withKey Laboratory for Anisotropy and Texture of Materials (MOE), Northeastern University
  • , Farzana DarainAffiliated withAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland Email author 
  • , Hui WangAffiliated withAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland
  • , Krassen DimitrovAffiliated withAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland

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Abstract

We report a simple and novel method for surface biofunctionalization onto recently reported Ni80Fe20 permalloy nanoparticles (~71 nm) and the immobilization of a model protein, IgG from human serum. The strategy of protein immobilization involved attachment of histidine-tagged streptavidin to the Ni80Fe20 nanoparticles via a non-covalent ligand binding followed by biotinylated human IgG binding on the nanoparticle surface using the specific high affinity avidin–biotin interaction. The biofunctionalization of Ni80Fe20 permalloy nanoparticles was confirmed by Fourier Transform InfraRed (FTIR) spectroscopy and protein denaturing gel electrophoresis (lithium dodecyl sulfate-polyacrylamide gel electrophoresis, LDS-PAGE). This protocol for surface functionalization of the novel nanometer-sized Ni80Fe20 permalloy particles with biological molecules could open diverse applications in disease diagnostics and drug delivery.

Keywords

Ni80Fe20 permalloy Magnetic nanoparticles Protein immobilization Avidin–biotin bridge Gel electrophoresis Nanomedicine