Brief Communication

Journal of Nanoparticle Research

, Volume 13, Issue 1, pp 39-43

Preparation of amino-modified titanate nanotubes and its striking adsorption ability to duplex DNA

  • Hongyun NiuAffiliated withState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Science, Chinese Academy of Sciences
  • , Yaqi CaiAffiliated withState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Science, Chinese Academy of Sciences Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

In this study, amino-modified titanate nanotubes (NH2-TNs) were prepared and used to adsorb duplex DNA. Macroscopic complexes between DNA and NH2-TNs were observed. The uptake of DNA by NH2-TNs was very fast; equilibrium was reached within 10 min at pH 3.6–5.0. The equilibrium capacity in the adsorption isotherm of NH2-TNs was 240 mg g−1, and further increase of the adsorption capacity was obtained with the addition of Ca2+ in solution. The adsorbed DNA could be released with phosphate buffer saline (PBS) solution without damage of the DNA strands. NH2-TNs could protect DNA from cleavage efficiently in the presence of DNase I.

Keywords

Amino-modified titanate nanotubes DNA adsorption Macroscopic complexes