Special focus: Safety of Nanoparticles

Journal of Nanoparticle Research

, Volume 12, Issue 1, pp 75-82

Dispersion of multi-walled carbon nanotubes in biocompatible dispersants

  • J.-P. PiretAffiliated withLaboratory of Biochemistry and Cellular Biology (URBC), University of Namur
  • , S. DetricheAffiliated withLaboratory of Chemistry and Electrochemistry of Surfaces, University of Namur
  • , R. VigneronAffiliated withLaboratory of Analysis by Nuclear Reactions (LARN), University of Namur
  • , S. VankoningslooAffiliated withLaboratory of Biochemistry and Cellular Biology (URBC), University of Namur
  • , S. RolinAffiliated withLaboratory of Pharmacy, University of Namur
  • , J. H. Mejia MendozaAffiliated withLaboratory of Chemistry and Electrochemistry of Surfaces, University of Namur
  • , B. MasereelAffiliated withLaboratory of Pharmacy, University of Namur
  • , S. LucasAffiliated withLaboratory of Analysis by Nuclear Reactions (LARN), University of Namur
  • , J. DelhalleAffiliated withLaboratory of Chemistry and Electrochemistry of Surfaces, University of Namur
    • , F. LuiziAffiliated withNanocyl s.a.
    • , C. SaoutAffiliated withLaboratory of Biochemistry and Cellular Biology (URBC), University of Namur
    • , O. ToussaintAffiliated withLaboratory of Biochemistry and Cellular Biology (URBC), University of Namur Email author 

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Abstract

Owing to their phenomenal electrical and mechanical properties, carbon nanotubes (CNT) have been an area of intense research since their discovery in 1991. Different applications for these nanoparticles have been proposed, among others, in electronics and optics but also in the medical field. In parallel, emerging studies have suggested potential toxic effects of CNT while others did not, generating some conflicting outcomes. These discrepancies could be, in part, due to different suspension approaches used and to the agglomeration state of CNT in solution. In this study, we described a standardized protocol to obtain stable CNT suspensions, using two biocompatible dispersants (Pluronic F108 and hydroxypropylcellulose) and to estimate the concentration of CNT in solution. CNT appear to be greatly individualized in these two dispersants with no detection of remaining bundles or agglomerates after sonication and centrifugation. Moreover, CNT remained perfectly dispersed when added to culture medium used for in vitro cell experiments. We also showed that Pluronic F108 is a better dispersant than hydroxypropylcellulose. In conclusion, we have developed a standardized protocol using biocompatible surfactants to obtain reproducible and stable multi-walled carbon nanotubes suspensions which can be used for in vitro or in vivo toxicological studies.

Keywords

Multi-walled CNT Pluronic F108 HPC Sonication Centrifugation Health safety Nanomedicine EHS