Journal of Nanoparticle Research

, Volume 12, Issue 1, pp 75–82

Dispersion of multi-walled carbon nanotubes in biocompatible dispersants

  • J.-P. Piret
  • S. Detriche
  • R. Vigneron
  • S. Vankoningsloo
  • S. Rolin
  • J. H. Mejia Mendoza
  • B. Masereel
  • S. Lucas
  • J. Delhalle
  • F. Luizi
  • C. Saout
  • O. Toussaint
Special focus: Safety of Nanoparticles

DOI: 10.1007/s11051-009-9697-8

Cite this article as:
Piret, J., Detriche, S., Vigneron, R. et al. J Nanopart Res (2010) 12: 75. doi:10.1007/s11051-009-9697-8

Abstract

Owing to their phenomenal electrical and mechanical properties, carbon nanotubes (CNT) have been an area of intense research since their discovery in 1991. Different applications for these nanoparticles have been proposed, among others, in electronics and optics but also in the medical field. In parallel, emerging studies have suggested potential toxic effects of CNT while others did not, generating some conflicting outcomes. These discrepancies could be, in part, due to different suspension approaches used and to the agglomeration state of CNT in solution. In this study, we described a standardized protocol to obtain stable CNT suspensions, using two biocompatible dispersants (Pluronic F108 and hydroxypropylcellulose) and to estimate the concentration of CNT in solution. CNT appear to be greatly individualized in these two dispersants with no detection of remaining bundles or agglomerates after sonication and centrifugation. Moreover, CNT remained perfectly dispersed when added to culture medium used for in vitro cell experiments. We also showed that Pluronic F108 is a better dispersant than hydroxypropylcellulose. In conclusion, we have developed a standardized protocol using biocompatible surfactants to obtain reproducible and stable multi-walled carbon nanotubes suspensions which can be used for in vitro or in vivo toxicological studies.

Keywords

Multi-walled CNTPluronic F108HPCSonicationCentrifugationHealth safetyNanomedicineEHS

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • J.-P. Piret
    • 1
  • S. Detriche
    • 4
  • R. Vigneron
    • 2
  • S. Vankoningsloo
    • 1
  • S. Rolin
    • 3
  • J. H. Mejia Mendoza
    • 4
  • B. Masereel
    • 3
  • S. Lucas
    • 2
  • J. Delhalle
    • 4
  • F. Luizi
    • 5
  • C. Saout
    • 1
  • O. Toussaint
    • 1
  1. 1.Laboratory of Biochemistry and Cellular Biology (URBC)University of NamurNamurBelgium
  2. 2.Laboratory of Analysis by Nuclear Reactions (LARN)University of NamurNamurBelgium
  3. 3.Laboratory of PharmacyUniversity of NamurNamurBelgium
  4. 4.Laboratory of Chemistry and Electrochemistry of SurfacesUniversity of NamurNamurBelgium
  5. 5.Nanocyl s.a.SambrevilleBelgium