Journal of Nanoparticle Research

, Volume 12, Issue 5, pp 1599–1608

Synthesis of bombesin-functionalized iron oxide nanoparticles and their specific uptake in prostate cancer cells

  • Amanda L. Martin
  • Jennifer L. Hickey
  • Amber L. Ablack
  • John D. Lewis
  • Leonard G. Luyt
  • Elizabeth R. Gillies
Research Paper

DOI: 10.1007/s11051-009-9681-3

Cite this article as:
Martin, A.L., Hickey, J.L., Ablack, A.L. et al. J Nanopart Res (2010) 12: 1599. doi:10.1007/s11051-009-9681-3

Abstract

The imaging of molecular markers associated with disease offers the possibility for earlier detection and improved treatment monitoring. Receptors for gastrin-releasing peptide are overexpressed on prostate cancer cells offering a promising imaging target, and analogs of bombesin, an amphibian tetradecapeptide have been previously demonstrated to target these receptors. Therefore, the pan-bombesin analog [β-Ala11, Phe13, Nle14]bombesin-(7–14) was conjugated through a linker to dye-functionalized superparamagnetic iron oxide nanoparticles for the development of a new potential magnetic resonance imaging probe. The peptide was conjugated via click chemistry, demonstrating a complementary alternative methodology to conventional peptide-nanoparticle conjugation strategies. The peptide-functionalized nanoparticles were then demonstrated to be selectively taken up by PC-3 prostate cancer cells relative to unfunctionalized nanoparticles and this uptake was inhibited by the presence of free peptide, confirming the specificity of the interaction. This study suggests that these nanoparticles have the potential to serve as magnetic resonance imaging probes for the detection of prostate cancer.

Keywords

Iron oxide Nanoparticles Bombesin Prostate cancer Magnetic resonance imaging Nanomedicine 

Supplementary material

11051_2009_9681_MOESM1_ESM.pdf (140 kb)
Supplementary material 1 (PDF 139 kb)

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Amanda L. Martin
    • 1
  • Jennifer L. Hickey
    • 1
  • Amber L. Ablack
    • 3
  • John D. Lewis
    • 3
    • 5
  • Leonard G. Luyt
    • 1
    • 3
    • 4
  • Elizabeth R. Gillies
    • 1
    • 2
  1. 1.Department of ChemistryThe University of Western OntarioLondonCanada
  2. 2.Department of Chemical and Biochemical EngineeringThe University of Western OntarioLondonCanada
  3. 3.Department of OncologyThe University of Western OntarioLondonCanada
  4. 4.Department of Medical ImagingThe University of Western OntarioLondonCanada
  5. 5.Department of Medical BiophysicsThe University of Western OntarioLondonCanada

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