Research Paper

Journal of Nanoparticle Research

, Volume 11, Issue 7, pp 1777-1785

Nanosized cancer cell-targeted polymeric immunomicelles loaded with superparamagnetic iron oxide nanoparticles

  • Rishikesh M. SawantAffiliated withDepartment of Pharmaceutical Sciences, Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University
  • , Rupa R. SawantAffiliated withDepartment of Pharmaceutical Sciences, Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University
  • , Evin GultepeAffiliated withElectronic Materials Research Institute, Northeastern University
  • , Dattatri NageshaAffiliated withElectronic Materials Research Institute, Northeastern University
  • , Brigitte Papahadjopoulos-SternbergAffiliated withNano Analytical Laboratory
  • , Srinivas SridharAffiliated withElectronic Materials Research Institute, Northeastern University
  • , Vladimir P. TorchilinAffiliated withDepartment of Pharmaceutical Sciences, Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University Email author 

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Abstract

Stable 30–50 nm polymeric polyethylene glycol–phosphatidylethanolamine (PEG–PE)-based micelles entrapping superparamagnetic iron oxide nanoparticles (SPION) have been prepared. At similar concentrations of SPION, the SPION-micelles had significantly better magnetic resonance imaging (MRI) T2 relaxation signal compared to ‘plain’ SPION. Freeze-fracture electron microscopy confirmed SPION entrapment in the lipid core of the PEG–PE micelles. To enhance the targeting capability of these micelles, their surface was modified with the cancer cell-specific anti-nucleosome monoclonal antibody 2C5 (mAb 2C5). Such mAb 2C5-SPION immunomicelles demonstrated specific binding with cancer cells in vitro and were able to bring more SPION to the cancer cells thus demonstrating the potential to be used as targeted MRI contrast agents for tumor imaging.

Keywords

Superparamagnetic iron oxide nanoparticles Polymeric micelles Immunomicelles Cancer-specific monoclonal antibody MRI contrast agent Nanomedicine