Journal of Nanoparticle Research

, Volume 12, Issue 1, pp 227–235

Electrochemical analysis of gold-coated magnetic nanoparticles for detecting immunological interaction

Authors

  • Thao Thi-Hien Pham
    • Nano-optics & Biomolecular Engineering National Laboratory, Department of Chemical EngineeringSungkyunkwan University
    • Nano-optics & Biomolecular Engineering National Laboratory, Department of Chemical EngineeringSungkyunkwan University
Research Paper

DOI: 10.1007/s11051-009-9600-7

Cite this article as:
Pham, T.T. & Sim, S.J. J Nanopart Res (2010) 12: 227. doi:10.1007/s11051-009-9600-7

Abstract

An electrochemical impedance immunosensor was developed for detecting the immunological interaction between human immunoglobulin (IgG) and protein A from Staphylococcus aureus based on the immobilization of human IgG on the surface of modified gold-coated magnetic nanoparticles. The nanoparticles with an Au shell and Fe oxide cores were functionalized by a self-assembled monolayer of 11-mercaptoundecanoic acid. The electrochemical analysis was conducted on the modified magnetic carbon paste electrodes with the nanoparticles. The magnetic nanoparticles were attached to the surface of the magnetic carbon paste electrodes via magnetic force. The cyclic voltammetry technique and electrochemical impedance spectroscopy measurements of the magnetic carbon paste electrodes coated with magnetic nanoparticles–human IgG complex showed changes in its alternating current (AC) response both after the modification of the surface of the electrode and the addition of protein A. The immunological interaction between human IgG on the surface of the modified magnetic carbon paste electrodes and protein A in the solution could be successfully monitored.

Keywords

Gold-coated magnetic nanoparticles Electrochemical impedance spectroscopy Cyclic voltammetry Immunological interaction Nanomedicine

Copyright information

© Springer Science+Business Media B.V. 2009