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Immunological impact of magnetic nanoparticles (Ferucarbotran) on murine peritoneal macrophages

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Abstract

Ferucarbotran, a clinically used superparamagnetic iron oxide, is widely developed as a magnetic resonance imaging (MRI) contrast agent and has the potential to improve the monitoring of macrophage recirculation in vivo. However, the biological effect of Ferucarbotran or magnetic nanoparticles (MNPs) on macrophage is not clearly understood yet. This study is aimed to examine the immunological impact of Ferucarbotran toward murine peritoneal macrophages. Cells treated with Ferucarbotran demonstrated a dose–responsive increase of granularity in the cytoplasm. After 24 h of incubation, viability and cytotoxicity in macrophages treated with 200 μg Fe/mL of Ferucarbotran were not affected. Macrophages loaded with Ferucarbotran above 100 μg Fe/mL showed a significant (p < 0.01) increase in cytokine (TNF-α, IL-1β, IL-6) secretion and mRNA expression, followed by nitric oxide (NO) secretion and iNOS mRNA expression. Chemotactic responses of Ferucarbotran-preloaded macrophages toward CX3CL1 were significantly (p < 0.05) lower than those of untreated macrophages. Taking together, Ferucarbotran at high dose (100 μg Fe/mL) could induce murine peritoneal macrophages activation in pro-inflammatory cytokine secretion and NO production.

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Abbreviations

MNPs:

Magnetic nanoparticles

MRI:

Magnetic resonance imaging

SPIO:

Superparamagnetic iron oxide

USPIO:

Ultrasmall superparamagnetic iron oxide

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Acknowledgments

This study was supported by grants from the National Science Council, Taiwan (NSC-96-2815-C-002-080-B).

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Correspondence to Fuu Sheu.

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Yeh, CH., Hsiao, JK., Wang, JL. et al. Immunological impact of magnetic nanoparticles (Ferucarbotran) on murine peritoneal macrophages. J Nanopart Res 12, 151–160 (2010). https://doi.org/10.1007/s11051-009-9589-y

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  • DOI: https://doi.org/10.1007/s11051-009-9589-y

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