Research Paper

Journal of Nanoparticle Research

, Volume 12, Issue 1, pp 151-160

First online:

Immunological impact of magnetic nanoparticles (Ferucarbotran) on murine peritoneal macrophages

  • Chen-Hao YehAffiliated withDepartment of Horticulture, National Taiwan University
  • , Jong-Kai HsiaoAffiliated withDepartment of Medical Imaging, National Taiwan University Hospital and College of Medicine
  • , Jaw-Lin WangAffiliated withInstitute of Biomedical Engineering, National Taiwan University
  • , Fuu SheuAffiliated withDepartment of Horticulture, National Taiwan UniversityResearch Center of Food and Biomolecules, National Taiwan University Email author 

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Ferucarbotran, a clinically used superparamagnetic iron oxide, is widely developed as a magnetic resonance imaging (MRI) contrast agent and has the potential to improve the monitoring of macrophage recirculation in vivo. However, the biological effect of Ferucarbotran or magnetic nanoparticles (MNPs) on macrophage is not clearly understood yet. This study is aimed to examine the immunological impact of Ferucarbotran toward murine peritoneal macrophages. Cells treated with Ferucarbotran demonstrated a dose–responsive increase of granularity in the cytoplasm. After 24 h of incubation, viability and cytotoxicity in macrophages treated with 200 μg Fe/mL of Ferucarbotran were not affected. Macrophages loaded with Ferucarbotran above 100 μg Fe/mL showed a significant (p < 0.01) increase in cytokine (TNF-α, IL-1β, IL-6) secretion and mRNA expression, followed by nitric oxide (NO) secretion and iNOS mRNA expression. Chemotactic responses of Ferucarbotran-preloaded macrophages toward CX3CL1 were significantly (p < 0.05) lower than those of untreated macrophages. Taking together, Ferucarbotran at high dose (100 μg Fe/mL) could induce murine peritoneal macrophages activation in pro-inflammatory cytokine secretion and NO production.


Superparamagnetic iron oxide Magnetic resonance imaging Cytotoxicity Inflammation Cell activation Chemotaxis Nanomedicine