Journal of Nanoparticle Research

, Volume 11, Issue 4, pp 821–829

Avidin conjugation to up-conversion phosphor NaYF4:Yb3+, Er3+ by the oxidation of the oligosaccharide chains

  • Deyan Kong
  • Zewei Quan
  • Jun Yang
  • Piaoping Yang
  • Chunxia Li
  • Jun Lin
Research Paper

DOI: 10.1007/s11051-008-9437-5

Cite this article as:
Kong, D., Quan, Z., Yang, J. et al. J Nanopart Res (2009) 11: 821. doi:10.1007/s11051-008-9437-5

Abstract

NaYF4:Yb3+, Er3+ nanoparticles were successfully prepared by a polyol process using diethyleneglycol (DEG) as solvent. After being functionalized with SiO2–NH2 layer, these NaYF4:Yb3+, Er3+ nanoparticles can conjugate with activated avidin molecules (activated by the oxidation of the oligosaccharide chain). The as-formed NaYF4:Yb3+, Er3+ nanoparticles, NaYF4:Yb3+, Er3+ nanoparticles functionalized with amino groups, avidin conjugated amino-functionalized NaYF4:Yb3+, Er3+ nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), atomic force microscopy (AFM), Fourier transform infrared (FT-IR), UV/Vis absorption spectra, and up-conversion luminescence spectra, respectively. The biofunctionalization of the NaYF4:Yb3+, Er3+ nanoparticles has less effect on their luminescence properties, i.e., they still show the up-conversion emission (from Er3+, with 4S3/2 → 4I15/2 at ~540 nm and 4F9/2 → 4I15/2 at ~653 nm), indicative of the great potential for these NaYF4:Yb3+, Er3+ nanoparticles to be used as fluorescence probes for biological system.

Keywords

Up-conversion phosphorAvidinOligosaccharide chainsFluorescence probeNanoparticlesBiomolecules

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Deyan Kong
    • 1
    • 2
  • Zewei Quan
    • 1
  • Jun Yang
    • 1
  • Piaoping Yang
    • 1
  • Chunxia Li
    • 1
  • Jun Lin
    • 1
  1. 1.State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchunPeople’s Republic of China
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingPeople’s Republic of China