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Mating Type Gene (MAT1-1) in Japanese Isolates of Trichophyton rubrum

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Abstract

Trichophyton rubrum is an anthropophilic species that is the most frequent etiologic agent of human dermatophytosis throughout the world. No teleomorph has been identified for T. rubrum strains. This study used PCR analysis to confirm the presence of a mating type locus in the genome of Japanese isolates of T. rubrum. To clarify the epidemiological and ecological characteristics of this fungus, mating type sequences were tested for correlation of MAT genotype to mating type. This study examined clinical isolates of T. rubrum that had been obtained from 206 human cases of tinea pedis and tinea unguium in Japan, including those from Fukuoka (29 strains), Gifu (23 strains), Kanazawa (63 strains), and Tokyo (91 strains), along with 10 isolates derived from 10 cases of canine dermatophytosis. PCR detected the presence of MAT1-1 in all of the human and animal isolates. Therefore, all isolates examined were expected to react as (−) type on the mating test and not as (+) type.

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Acknowledgments

This study was supported by Grants-in-Aid from the Academic Frontier Project of the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT), and Grants-in-Aid from Nihon University. We thank Dr. T. Kasai (Kasai Dermatological Hospital), Dr. T. Kusunoki (Kusunoki Dermatological Hospital), Dr. M. Fujihiro, (Department of Dermatology, Ibi General Hospital), and Dr. H. Noguchi (Noguchi Dermatological Hospital) for providing the clinical isolates used in this study.

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Authors and Affiliations

  1. Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Fujisawa, Kanagawa, 252-0880, Japan

    Rui Kano, Maiko Isizuka & Hiroshi Kamata

  2. Department of Dermatology and Allergology, Juntendo University Nerima Hospital, 3-1-10 Takanodai, Nerima, Tokyo, 177-0033, Japan

    Masataro Hiruma

  3. Department of Dermatology, Kanazawa Medical University, Uchinada, Ishikawa, 920-0293, Japan

    Takashi Mochizuki

  4. Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo, 192-0395, Japan

    Atsuhiko Hasegawa

Authors
  1. Rui Kano
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  2. Maiko Isizuka
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  3. Masataro Hiruma
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  4. Takashi Mochizuki
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  5. Hiroshi Kamata
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  6. Atsuhiko Hasegawa
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Correspondence to Rui Kano.

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Kano, R., Isizuka, M., Hiruma, M. et al. Mating Type Gene (MAT1-1) in Japanese Isolates of Trichophyton rubrum . Mycopathologia 175, 171–173 (2013). https://doi.org/10.1007/s11046-012-9603-2

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  • DOI: https://doi.org/10.1007/s11046-012-9603-2

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