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Jeju ground water containing vanadium induces normal T cell development and immune activation in chronically stressed mice

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Abstract

Containing high concentration of vanadium served by the volcanic bedrock, Jeju ground water has long been known for various implicit health benefits including immune-promotion. Exposure to stress has been reported to be associated with immunosuppression such as reducing lymphocyte population or antibody production due to stress hormones. In this study, we aimed at evaluating the effects of Jeju ground water on chronically stressed mice. C57BL/6 mice were subjected to various stressors such as restraint stress, water swimming stress, heat stress, acoustic stress, and Jeju ground water was supplied for 28 days with two different concentrations, S1 (vanadium 15–20 μg/l, pH 8.3) and S2 (vanadium 20–25 μg/l, pH 8.5). Treatment with Jeju ground water increased CD4+CD8− or CD4−CD8+ single-positive thymocytes. It also increased the proliferation of splenocytes and the populations of CD4+ T cells, CD45R/B220+ B cells, CD11b+ macrophages or Gr-1+ granulocytes in spleen. In addition, the production of IgG was increased in chronically stressed mice by treatment with Jeju ground water. These results suggest vanadium-rich Jeju ground water may be helpful in T cell development in thymus and immune cell proliferation and its function in spleen against chronic stress.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

ACK:

Ammonium chloride potassium

ANOVA:

One-way analysis of variance

AVP:

Arginine vasopressin

CD:

Cluster of differentiation

Con A:

Concanavalin A

CRH:

Corticotropin-releasing hormone

DPBS:

Dulbecco’s phosphate-buffered saline

ELISA:

Enzyme linked immunosorbent assay

FBS:

Fetal bovine serum

HPA axis:

Hypothalamic pituitary adrenal axis

NF-κB:

Nuclear factor kappa beta

IL:

Interleukin

Ig:

Immunoglobulin

SE:

Standard error

Th:

T helper

TW:

Tap water

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Acknowledgements

The authors thank Dr. T.H. Chung for editorial assistance.

Funding

This research was financially supported by the Jeju Special Self-Governing Province Development Corporation (JPDC).

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Authors and Affiliations

  1. College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, 102 Jejudaehakno, Jeju, 63243, Republic of Korea

    Jinhee Cho, So Jin Bing & Youngheun Jee

  2. Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, 20892, USA

    So Jin Bing

  3. Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju, 63243, Republic of Korea

    Areum Kim, Kalahe Hewage Iresha Nadeeka Madushani Herath & Youngheun Jee

  4. Department of Food Technology and Nutrition, Chonnam National University, Yeosu, 59626, Republic of Korea

    Ginnae Ahn

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  1. Jinhee Cho
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Contributions

JC, SJB, AK, KHINMH performed experiments and analyzed data. GA helped to interpret the data and improve the manuscript. JC drafted the manuscript. YJ conceived of the study, designed the research and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Youngheun Jee.

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The authors declare that they have no competing interests.

Ethical approval

All animal experiments were conducted in accordance with the Jeju National University Guide for the Care and Use of Laboratory Animals and were approved by the Animal Care and Use Committee of Jeju National University.

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Cho, J., Bing, S.J., Kim, A. et al. Jeju ground water containing vanadium induces normal T cell development and immune activation in chronically stressed mice. Mol Biol Rep 46, 4443–4452 (2019). https://doi.org/10.1007/s11033-019-04899-0

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