Skip to main content
SpringerLink
Log in

RNAi mediated gene silencing against betasatellite associated with Croton yellow vein mosaic begomovirus

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Plant viruses encode suppressors of posttranscriptional gene silencing, an adaptive antiviral defense responses that confines virus infection. Previously, we identified single-stranded DNA satellite (also known as DNA-β) of ~1,350 nucleotides in length associated with Croton yellow vein mosaic begomovirus (CYVMV) in croton plants. The expression of genes from DNA-β requires the begomovirus for packaged, replication, insect transmission and movement in plants. The present study demonstrates the effect of the βC1 gene on the silencing pathway as analysed by using both transgenic systems and transient Agrobacterium tumefaciens based delivery. Plants that carry an intron-hairpin construct covering the βC1 gene accumulated cognate small-interfering RNAs and remained symptom-free after exposure to CYVMV and its satellite. These results suggest that βC1 interferes with silencing mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Mansoor S, Briddon RW, Zafar Y, Stanley J (2003) Geminivirus disease complexes: an emerging threat. Trends Plant Sci 8:128–134

    Article  PubMed  CAS  Google Scholar 

  2. Bass BL (2000) Double-stranded RNA as a template for gene silencing. Cell 101:235–238

    Article  PubMed  CAS  Google Scholar 

  3. Saunders K, Norman A, Gucciardo S, Stanley J (2004) The DNA-β satellite component associated with ageratum yellow vein disease encodes an essential pathogenicity protein (βC1). Virology 324:37–47

    Article  PubMed  CAS  Google Scholar 

  4. Vaucheret H (2006) Post-transcriptional small RNA pathways in plants: mechanisms and regulations. Genes Dev 20:759–771

    Article  PubMed  CAS  Google Scholar 

  5. Ikegami M, Kon T, Sharma P (2011) RNA silencing and viral encoded silencing suppressors. In: Gaur RK, Gafni Y, Gupta VK, Sharma P (eds) RNAi technology. CRC Press, USA, pp 209–240

  6. Chapman EJ, Prokhnevsky AI, Gopinath K, Dolja VV, Carrington JC (2004) Viral RNA silencing suppressors inhibits the microRNA pathway at an intermediate step. Genes Dev 18:1179–1186

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  7. Mourrain P, Beclin C, Elmayan T, Feuerbach F, Godon C, Morel JB, Jouette D, Lacombe AM, Nikic S, Picault N, Remoue K, Sanial M, Vo TA, Vaucheret H (2000) Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance. Cell 101:533–542

    Article  PubMed  CAS  Google Scholar 

  8. Smith NA, Singh SP, Wang MB, Stoutjesdijk PA, Green AG, Waterhouse PM (2000) Gene expression: total silencing by intron-spliced hairpin. Nature 407:319–320

    Article  PubMed  CAS  Google Scholar 

  9. Vaistij FE, Jones L, Baulcombe DC (2002) Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase. Plant Cell 14:857–867

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  10. Vaucheret H, Fagard M (2001) Transcriptional gene silencing in plants: targets, inducers and regulators. Trends Genet 17:29–35

    Article  PubMed  CAS  Google Scholar 

  11. Zamore PD (2004) Plant RNAi: how a viral silencing suppressor inactivates siRNA. Curr Biol 14:R198–R200

    Article  PubMed  CAS  Google Scholar 

  12. Covey SN, Al-Kaff NS, Lángara A, Turner DS (1997) Plants combat infection by gene silencing. Nat (Lond) 385:781–782

    Article  CAS  Google Scholar 

  13. Ratcliff FG, Harrison BD, Baulcombe DC (1997) A similarity between viral defense and gene silencing in plants. Science 276:1558–1560

    Article  PubMed  CAS  Google Scholar 

  14. Saunders K, Bedford ID, Yahara T, Stanley J (2003) The earliest recorded plant virus disease. Nature 422:831

    Article  PubMed  CAS  Google Scholar 

  15. Zhou XP, Xie Y, Tao XR, Zhang ZK, Li ZH, Fauquet CM (2003) Characterization of DNA β associated with begomoviruses in China and evidence for co-evolution with their cognate viral DNA-A. J Gen Virol 84:237–247

    Article  PubMed  CAS  Google Scholar 

  16. Sijen T, Kooter JM (2000) Post-transcriptional gene-silencing: RNAs on the attack or on the defense. BioEssays 22:520–531

    Article  PubMed  CAS  Google Scholar 

  17. Nagel R, Elliot A, Masel A, Birch RG, Manners JM (1990) Electroporation of binary Ti plasmid vector into Agrobacterium tumefaciens and Agrobacterium rizogenes. FEMS Microbiol Lett 67:325–328

    Article  CAS  Google Scholar 

  18. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  19. Kon T, Dolores LM, Bajet NB, Hase S, Takahashi H, Ikegami M (2003) Molecular Characterization of a strain of squash leaf curl China virus from the Philippines. J Phytopathol 151:535–539

    Article  CAS  Google Scholar 

  20. Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, Malik KA, Markham PG (2001) Identification of DNA components required for induction of cotton leaf curl disease. Virology 285:234–243

    Article  PubMed  CAS  Google Scholar 

  21. Jose J, Usha R (2003) Bhendi yellow vein mosaic disease in India is caused by association of a DNAβ-satellite with a begomovirus. Virology 305:310–317

    Article  PubMed  CAS  Google Scholar 

  22. Hafner GJ, Stafford MR, Wolter LC, Harding RM, Dale JL (1997) Nicking and joining activity of banana bunchy top virus replication protein in vitro. J Gen Virol 78:1795–1799

    PubMed  CAS  Google Scholar 

  23. Bai QR, Zhu JH, Liu XL, Zhu CX, Song YZ, Wen FJ (2005) Production of transgenic tobacco plants resistant to two viruses via RNA-mediated virus resistance. Acta Phytopathol Sin 35:148–154

    Google Scholar 

  24. Jan FJ, Fagoaga C, Pang SZ, Gonsalves D (2000) A single chimeric transgene derived from two distinct viruses confers multi-virus resistance in transgenic plants through homology-dependent gene silencing. J Gen Virol 81:2103–2109

    PubMed  CAS  Google Scholar 

  25. Bucher E, Lohuis D, van Pieter M, Poppel JA, Geerts Dimitriadou C, Goldbach R, Prins M (2006) Multiple virus resistance at a high frequency using a single transgene construct. J Gen Virol 87:3697–3701

    Article  PubMed  CAS  Google Scholar 

  26. Zhu CX, Song YZ, Yin GH, Wen FJ (2009) Induction of RNA-mediated multiple virus resistance to Potato virus Y, Tobacco mosaic virus and Cucumber mosaic virus. J Phytopathol 157:101–107

    Article  CAS  Google Scholar 

  27. Pandolfini T, Molesini B, Avesani L, Spena A, Polverari A (2003) Expression of self-complementary hairpin RNA under the control of the rolC promoter confers systemic disease resistance to plum pox virus without preventing local infection. BMC Biotechnol 3:7–21

    Article  PubMed  PubMed Central  Google Scholar 

  28. Duan YP, Powell CA, Purcifull DE, Broglio P, Hiebert E (1997) Phenotypic variation in transgenic tobacco expressing mutated geminivirus movement/pathogenicity (BC1) proteins. Mol Plant Microbe Interact 10:1065–1074

    Article  PubMed  CAS  Google Scholar 

  29. Kunik T, Salomon R, Zamir D, Navot N, Zeidan M, Michelson I, Gafni Y, Czosnek H (1994) Transgenic tomato plants expressing the tomato yellow leaf curl virus capsid protein are resistant to the virus. Biotechnology (NY) 12:500–504

    Article  CAS  Google Scholar 

  30. Baulcombe D (2002) Viral suppression of systemic silencing. Trends Microbiol 10:306–308

    Article  PubMed  CAS  Google Scholar 

  31. Hamilton A, Voinnet O, Chappell L, Baulcombe D (2002) Two classes of short interfering RNA in RNA silencing. EMBO J 21:4671–4679

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  32. Mathieu O, Bender J (2004) RNA-directed DNA methylation. J Cell Sci 117:4881–4888

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors are thankful to Department of Science and Technology (DST Project no. SR/FT/LS-042/2009), and Department of Biotechnology (DBT Project no. BT/PR13129/GBD/27/197/2009), India for financial support. The authors are also grateful to Prof. Thomas Hohn, Switzerland, Dr. Indu Sharma, Dr. Ajay Kumar Chaubey and Dr. Narendra Kumar for their valuable suggestions.

Author information

Authors and Affiliations

  1. Department of Science, Faculty of Arts, Science and Commerce, Mody Institute of Technology and Science, Lakshmangarh, Sikar, 332311, India

    Anurag Kumar Sahu, Avinash Marwal, Chitra Nehra, Devendra Kumar Choudhary & Rajarshi Kumar Gaur

  2. Division of Crop Improvement, Directorate of Wheat Research, Karnal, 132 001, India

    Pradeep Sharma

Authors
  1. Anurag Kumar Sahu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Avinash Marwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chitra Nehra
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Devendra Kumar Choudhary
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pradeep Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rajarshi Kumar Gaur
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Pradeep Sharma or Rajarshi Kumar Gaur.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 31 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sahu, A.K., Marwal, A., Nehra, C. et al. RNAi mediated gene silencing against betasatellite associated with Croton yellow vein mosaic begomovirus . Mol Biol Rep 41, 7631–7638 (2014). https://doi.org/10.1007/s11033-014-3653-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-014-3653-0

Keywords

Search

Navigation