Abstract
Tobacco use and environmental air pollution are the established etiological factors in head and neck cancer (HNC) progression. Nevertheless, not all the inhabitants with high usage of tobacco from the same polluted locality are suffering with HNC and this is due to the existence of factors like inter-individual genetic polymorphisms, life time exposure to tobacco and the rate of xenobiotic metabolism enzyme (XME) activity. The present study investigates the polymorphic genotypes of the most important XME, glutathione-S-transferase Mu 1 (GST M1) and Theta 1 (GST T1) as the risk modulator to HNC among tobacco-habituated inhabitants of Saurashtra in Gujarat, a region in western India. A population based case–control study was done in 252 HNC patients and 504 healthy controls. Blood samples were collected from the subjects and investigated for polymorphic genotypes of GST M1 and GST T1. Estimation of the odds of risks was done by logistic regressions. Among the subjects with high usage of tobacco, M1 not null-T1 null genotypes presence was found as risk reducing factor to HNC with 0.334 folds (95 % CI; 0.170–0.659). The presence of M1 null-T1 not null genotypes was found with susceptibility to HNC among the subjects with no habit of tobacco chewing, adjusted odds ratio (AOR) 3.170 (1.128–8.913) and no habit of smoking, AOR of 2.544 (1.094–5.963). The present study reveals the finding of significantly increased risk to HNC by interactions of GST M1 null-GST T1 not null polymorphic genotypes among the subjects with nil or less tobacco usage shed some light for the insights of biomarker application in early detection of HNC.
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Li Q, Chuang S, Eluf-Neto J, Menezes A, Matos E, Koifman S et al (2012) Vitamin or mineral supplement intake and the risk of head and neck cancer: pooled analysis in the INHANCE consortium. Int J Cancer 131:1686–1699
Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM (2010) Estimates of worldwide burden of cancer in 2008:gLOBOCAN 2008. Int J Cancer 127:2893–2917
Sturgis EM, Cinciripini PM (2007) Trends in head and neck cancer incidence in relation to smoking prevalence. Cancer 110:1429–1435
Hecht SS (2006) Cigarette smoking: cancer risks, carcinogens, and mechanisms. Langenbecks Arch Surg 391:603–613
Dikshit R, Gupta PC, Ramasundarahettige C, Gajalakshmi V, Aleksandrowicz L, Badwe R et al (2012) Cancer mortality in India: a nationally representative survey. Lancet 379:1807–1816
Singh A, Kohli JS (2012) Effect of pollution on common man in India: a legal perspective. Adv Life Sci Technol 4:35–41
Rani M, Bonu S, Jha P, Nguyen SN, Jamjoum L (2003) Tobacco use in India: prevalence and predictors of smoking and chewing in a national cross sectional household survey. Tob Control. doi:10.1136/tc.12.4.e4
Ali I, Wani WA, Saleem K (2011) Cancer scenario in India with future perspectives. Cancer Ther 8:56–70
Hashibe M, Brennan P, Strange RC, Bhisey R, Cascorbi I, Lazarus P et al (2003) Meta- and pooled analyses of GSTM1, GSTT1, GSTP1, and CYP1A1 genotypes and risk of head and neck cancer. Cancer Epidemiol Biomark Prev 12:1509–1517
Canova C, Hashibe M, Simonato L, Nelis M, Metspalu A, Lagiou P et al (2009) Genetic associations of 115 polymorphisms with cancers of the upper aerodigestive tract across 10 European countries: the ARCAGE project. Cancer Res 7:2956–2965
Boysen G, Hecht SS (2003) Analysis of DNA and protein adducts of benzo[a]pyrene in human tissues using structure-specific methods. Mutat Res 543:17–30
Kriek E, Rojas M, Alexandrov K, Bartsch H (1998) Polycyclic aromatic hydrocarbon-DNA adducts in humans: relevance as biomarkers for exposure and cancer risk. Mutat Res 400:215–231
Chuang C, Tung J, Su M, Wu B, Hsin C, Chen Y et al (2012) BPDE-like DNA adduct level in oral tissue may act as a risk biomarker of oral cancer. Arch Oral Biol. doi:10.1016/j.archoralbio.2012.06.004
Hecht SS (2003) Tobacco carcinogens, their biomarkers and tobacco-induced cancer. Nat Rev Cancer 3:733–744
Phillips DH (2002) Smoking-related DNA and protein adducts in human tissues. Carcinogenesis 23:1979–2004
Tang D, Phillips DH, Stampfer M (2001) Association between Carcinogen-DNA adducts in white blood cells and lung cancer risk in the physicians health study. Cancer Res 61:6708–6712
Salah BG, Kallabi F, Maatoug S, Mkaouar-rebai E, Fourati A, Fakhfakh F et al (2012) Polymorphisms of glutathione S-transferases M1, T1, P1 and A1 genes in the Tunisian population: an intra and interethnic comparative approach. Gene 498:317–322
Ghosh T, Gupta S, Bajpai P, Agarwal D, Agarwal M, Gupta OP et al (2012) Association of CYP1A1, GSTM1, and GSTT1 gene polymorphism with risk of oral submucous fibrosis in a section of North Indian population. Mol Biol Rep 39:9383–9389
Hayes JD, Pulford DJ (1995) The glutathione S-transferases supergene family: regulation of GST and contribution of the isoenzymes to cancer chemoprevention and drug resistance. Crit Rev Biochem Mol Biol 31:445–460
Zhang Z, Hao K, Shi R, Zhao G, Jiang G, Song Y, Xu X et al (2011) Glutathione S-Transferase M1 (GSTM1) and Glutathione S-Transferase T1 (GSTT1) null polymorphisms, smoking, and their interaction in oral cancer: a HuGE review and meta-analysis. Am J Epidemiol 173:847–857
Tripathy CB, Roy N (2006) Meta-analysis of glutathione S-transferase M1 genotype and risk towards head and neck cancer. Head Neck 28:217–224
Anantharaman D, Chaubal PM, Kannan S, Bhisey RA, Mahimkumar MB (2007) Susceptibility to oral cancer by genetic polymorphisms at CYP1A1, GSTM1 and GSTT1 loci among Indians: tobacco exposure as a risk modulator. Carcinogenesis 28:1455–1462
Gupta PC, Murti PR, Bhonsle RB (1996) Epidemiology of cancer by tobacco products and the significance of TSNA. Crit Rev Toxicol 26:183–198
Lahiri DK, Nurnberger JI (1991) A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies. Nucl Acids Res 19:5444
Huang K, Sandler RS, Millikan RC, Schroeder JC, North KE, Hu J (2006) GSTM1 and GSTT1 polymorphisms, cigarette smoking, and risk of colon cancer: a population-based case-control study in North Carolina (United States). Cancer Causes Control 17:385–394
Moore LE, Huang W, Chatterjee N, Gunter M, Chanock S, Yeager M et al (2005) GSTM1, GSTT1, and GSTP1 polymorphisms and risk of advanced colorectal adenoma. Cancer Epidemiol Biomark Prev 14:1823–1827
Marur S, Forastiere AA (2008) Head and neck cancer: changing epidemiology, diagnosis, and treatment. Mayo Clin Proc 83:489–501
Chaung SC, Agudo A, Ahrens W, Anantharaman D, Benhamou S, Boccia S et al (2011) Sequence variants and the risk of head and neck cancer: pooled analysis in the INHANCE consortium. Front Oncol. doi:10.3389/fonc.2011.00013
Das SK, Balakrishnan V, Vasudevan DM (2006) Alcohol: its health and social impact in India. Natl Med J India 19:94–99
Ghassem-Fachandi P (2010) Ahimsa, identification and sacrifice in the Gujarat pogrom. Soc Anthropol 18:155–175
Senthilkumar KP, Thirumurugan R (2012) Impact of tobacco on glutathione S transferase gene loci of Indian ethnics. Asian Pacific J Cancer Prev 13:5037–5042
Sabitha K, Reddy MVV, Jamil K (2008) GST genotypes in head and neck cancer patients and its clinical implications. Afr J Biotechnol 7:3853–3859
Singh M, Shah PP, Singh AP, Ruwali M, Mathur N, Pant MC et al (2008) Association of genetic polymorphisms in glutathione S-transferases and susceptibility to head and neck cancer. Mutat Res 638:184–194
Shukla D, Kale AD, Hallikerimath S, Vivekanandhan S, Venkatakanthaiah Y (2012) Genetic polymorphism of drug metabolizing enzymes (GSTM1 and CYP1A1) as risk factors for oral premalignant lesions and oral cancer. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 156:253–259
Garcia-Closas M, Kelsey KT, Hankinson SE, Spiegelman D, Springer K, Willett WC et al (1999) Glutathione S-transferase mu and theta polymorphisms and breast cancer susceptibility. J Natl Cancer Inst 91:1960–1964
Kim WJ, Kim H, Kim CH, Lee MS, Oh BR, Lee HM, Katoh T (2002) GSTT1-null genotype is a protective factor against bladder cancer. Urology 60:913–918
Senthilkumar KP, Thirumurugan R (2012) GSTM1 and GSTT1 allele frequencies among various Indian and non-Indian ethnic groups. Asian Pac J Cancer Prev 13:6263–6267
Hung HC, Chuang J, Chien YC, Chern HD, Chaing C, Kuo Y et al (1997) Genetic polymorphisms of CYP2E1, GSTM1, and GSTT1; environmental factors and risk of oral cancer. Cancer Epidemiol Biomark Prev 6:901–905
Landi S (2000) Mammalian class theta GST and differential susceptibility to carcinogens: a review. Mutat Res 463:247–283
Zhang Y, Yn Ni, Zhang H, Pan Y, Ma Ju, Wang L (2012) Association between GSTM1 and GSTT1 allelic variants and head and neck squamous cell cancinoma. PLoS ONE 10:e47579
Hoffmann AA, Willi Y (2008) Detecting genetic responses to environmental change. Nat Rev Genet 9:421–432
Acknowledgments
We thank the participants of the study for their cooperation. We thank Dr. V. K. Gupta, Director, Shree Gulabkuvar Talakchand Sheth Cancer hospital, Rajkot for providing technical guidance. Authors are grateful to Dr. Balasubramani K. Goundappa, Graduate School of Public Health, University of Pittsburgh, USA for critical analyses of the data. The authors wish to thank Shree M. and N. Virani Science College, Rajkot, Gujarat, India and Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India for providing the support in sample collection and facilities.
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Senthilkumar, K.P., Thirumurugan, R. Risk modulation of GSTM1–GSTT1 interactions to head and neck cancer in tobacco users. Mol Biol Rep 41, 5635–5644 (2014). https://doi.org/10.1007/s11033-014-3433-x
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DOI: https://doi.org/10.1007/s11033-014-3433-x