Abstract
Abnormal cell proliferation is a main driver of tumor formation and development, which involves the deletion, mutation, and downregulation of tumor suppressor genes. One study recently demonstrated that miR-200a plays an oncogenic role by inhibiting phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression. In the human endometrial adenocarcinoma cell line HEC-1B, suppression of miR-200a expression inhibited cell proliferation and promoted apoptosis, whereas its over-expression had no effect on proliferation and apoptosis. Furthermore, inhibition or over-expression of miR-200a increased or reduced the expression of PTEN, respectively, with no change in PTEN mRNA levels. These effects were achieved by directly targeting miR-200a to the 3′ untranslated region of the PTEN mRNA to inhibit its translation. Taken together, we propose that in HEC-1B cells, miR-200a functions as an oncogene, affecting proliferation and apoptosis by regulating the expression of the tumor suppressor PTEN at the translational level.
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Acknowledgments
We thank Prof. Yingxiong Wang for helpful suggestions, and Prof. Junlin He for assistance with the statistical analysis. This study was supported by the National Natural Science Foundation of China (Grant number 31271545). The National Key Clinical Department Funding (201101CKZD). The foundation played no role in the study’s design, the collection or analysis of data, the decision to publish, or the preparation of the manuscript.
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Li, R., He, Jl., Chen, Xm. et al. MiR-200a is involved in proliferation and apoptosis in the human endometrial adenocarcinoma cell line HEC-1B by targeting the tumor suppressor PTEN. Mol Biol Rep 41, 1977–1984 (2014). https://doi.org/10.1007/s11033-014-3045-5
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DOI: https://doi.org/10.1007/s11033-014-3045-5