Abstract
The objective of this research were to detect bovine Dickkopf 2 (DKK2) gene polymorphism and analyze their associations with body measurement traits (BMT) and meat quality traits (MQT) of animals. Blood samples were taken from a total of 541 Qinchuan cattle aged from 18 to 24 months. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was employed to find out DKK2 single-polymorphism nucleotide (SNPs) and to explore their possible association with BMT and MQT. Sequence analysis of DKK2 gene revealed 2 SNPs (C29 T and A169C) in 5′ untranslated region (5′UTR) of exon 1.C29T and A164T SNPs are both synonymous mutation, which showed 2 genotypes namely (CC, CT) and (AA and AC), respectively. Association analysis of polymorphism with body measurement and meat quality traits at the two locus showed that there were significant effects on CT, BL, RL, PBW, BFT, LMA, and IFC. These results suggest that the DKK2 gene might have potential effects on BMT and MQT in Qinchuan cattle population and could be used for marker-assisted selection.
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References
Angela M, Inzerillo E (2004) Osteoporosis and diabetes mellitus. Rev Endocr Metab Disord 53:261–268
Westendorf JJ, Kahler RA, Schroeder TM (2004) Wnt signaling in osteoblasts and bone diseases. Gene 341:19–39
Logan CY, Nusse R (2004) The Wnt signaling pathway in development and disease. Annu Rev Cell Dev Biol 20:781–810
Kawano K (2003) Secreted antagonists of the Wnt signalling pathway. J Cell Sci 116:2627–2634
Valery E, John D (1999) Functional and structural diversity of the human Dickkopf gene family. Gene 238:301–313
Rawadi G, Roman-Roman S (2005) Wnt signalling pathway: a new target for the treatment of osteoporosis. Exp Opin Ther Targets 9:1063–1077
Niehrs C (2006) Function and biological roles of the Dickkopf family of Wnt modulators. Oncogene 25:7469–7481
Hausler KD (2004) Secreted frizzled-related protein-1 inhibits RANKL-dependent osteoclast formation. J Bone Miner Res 19:1873–1881
Li X (2005) Dkk2 has a role in terminal osteoblast differentiation and mineralized matrix formation. Nat Genet 37:945–952
Bodine PV (2004) The Wnt antagonist secreted frizzled-related protein-1 is a negative regulator of trabecular bone formation in adult mice. Mol Endocrinol 18:1222–1237
Gilbert RP, Bailey DRC, Shannon NH (1993) Linear body measurements of cattle before and after 20 years of selection for postweaning gain when fed two different diets. J Anim Sci 71:1712–1720
Hamlin KE, Green RD, Cundiff LV (1995) Real-time ultrasonic measurement of fat thickness and longissimus muscle area: II. Relationship between real-time ultrasound measures and carcass retail yield. J Anim Sci 73:1725–1734
Brethour JR (1994) Estimating marbling score in live cattle from ultrasound images using pattern recognition and neural network procedures. J Anim Sci 72:1425–1432
Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt-chloroform extraction of DNA from blood and tissues. Trends Genet 5(12):391
Nei M, Roychoudhury AK (1974) Sampling variance of heterozygosity and genetic distance. Genetics 76:379–390
Zhang CL, Wang Y, Chen H, Lan XY (2007) Enhance the efficiency of single-strand conformation polymorphism analysis by short polyacrylamide gel and modified silver staining. Anal Biochem 365:286–287
Gong Y (2001) LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development. Cell 107:513–523
Ohnaka K, Tanabe M (2005) Glucocorticoid suppresses the canonical Wnt signal in cultured human osteoblasts. Biochem Biophys Res Commun 329:177–181
Wang FS (2005) Secreted frizzled-related protein 1 modulates glucocorticoid attenuation of osteogenic activities and bone mass. Endocrinology 146:2415–2423
Li X (2005) Dkk2 has a role in terminal osteoblast differentiation and mineralized matrix formation. Nat Genet 37:945–952
Rothschild MF, Soller M (1997) Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe Newsl Agric Genomic 8:13–20
Hausler KD (2004) Secreted frizzled-related protein-1 inhibits RANKL-dependent osteoclast formation. J Bone Miner Res 19:1873–1881
Li F, Chen H, Lei CZ (2010) Novel SNPs of the bovine GAD1/gad67 gene and their association with growth traits in three native Chinese cattle breeds. Mol Biol Rep 37:501–505
Chu MX, Zhao XH, Zhang YJ, Jin M et al (2010) Polymorphisms of BMPR-IB gene and their relationship with litter size in goats. Mol Biol Rep 37(8):4033–4039
Schulz RA, Yutzey KE (2004) Calcineurin signaling and NFAT activation in cardiovascular and skeletal muscle development. Dev Biol. 266:1–16
Babij P (2003) High bone mass in mice expressing a mutant LRP5 gene. J Bone Miner Res 18:960–974
Li X (2005) Dkk2 has a role in terminal osteoblast differentiation and mineralized matrix formation. Nat Genet 37:945–952
Acknowledgments
We thank all the research assistants and laboratory technicians who contributed to this study. We extend special thanks to Professor Zan and Dr. Cheng for their assistance. This work was supported by Program for Changjiang Scholars and Innovative Research Team (IRT0940), “13115” Scientific and Technological Innovation Program of Shaanxi Province (S2010ZDGC109) and GMO new varieties major project (2008ZX08007-002).
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Zhan, X., Gao, J., HuangFu, Y. et al. Polymorphisms of the bovine DKK2 and their associations with body measurement traits and meat quality traits in Qinchuan cattle. Mol Biol Rep 40, 6831–6835 (2013). https://doi.org/10.1007/s11033-013-2800-3
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DOI: https://doi.org/10.1007/s11033-013-2800-3