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Gender specificity of a genetic variant of angiotensin-converting enzyme and risk of coronary artery disease

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Abstract

Etiological factors for coronary artery disease (CAD) involve a wide range of gene and environmental interactions. One of the systems being implicated in the pathophysiology of CAD is the renin-angiotensin system (RAS). However, the genetic polymorphisms of this system have not been widely studied in Iranian patients diagnosed with CAD. The aim of this study was to assess the relationship between six gene polymorphisms of RAS components and CAD in a sample of Iranian population. A total of 374 participants were enrolled in a case/control study. The presence of CAD was determined by coronary angiography. Genotyping of six RAS gene polymorphisms was performed using a modified PCR–RFLP method. Our results revealed, for the first time, a significant independent association of angiotensin-converting enzyme (ACE) A-240T polymorphism and incidence of CAD among Iranian women (P = 0.005, OR = 20.4, 95 % CI = 2.49–41.2). There has also been a significant difference in genotype distribution of ACE A-240T (P = 0.008) and angiotensin II receptor type 2 C3123A polymorphism (P = 0.032) in Iranian female participants. In conclusion, TT genotype of ACE A-240T seems to be a genetic risk factor for CAD in Iranian women.

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Acknowledgments

This study was a part of a PhD thesis supported by Tehran University of Medical Sciences (Grant No: P-843). The authors would like to thank Ms. Shohreh Hekmat for her assistance in biochemical assays and Mrs. Askari for helping in sample collection.

Conflict of interest

The authors report no conflicts of interest and they are alone responsible for the content and writing of the paper.

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Authors and Affiliations

  1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Hemmat Highway, 14496, Tehran, Iran

    Negar Firouzabadi & Massoumeh Shafiei

  2. Division of Transplant Immunology and Immunogenetics, Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Nader Tajik

  3. Department of Cardiology and Cardiovascular Research, Rajaee Heart Center, Tehran University of Medical Sciences, Tehran, Iran

    Ehsan Bahramali & Mohsen Maadani

  4. Department of Epidemiology and Biostatistics, Rajaee Heart Center, Tehran University of Medical Sciences, Tehran, Iran

    Hooman Bakhshandeh

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  1. Negar Firouzabadi
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  2. Nader Tajik
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  3. Ehsan Bahramali
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  4. Hooman Bakhshandeh
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  5. Mohsen Maadani
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  6. Massoumeh Shafiei
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Correspondence to Massoumeh Shafiei.

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Firouzabadi, N., Tajik, N., Bahramali, E. et al. Gender specificity of a genetic variant of angiotensin-converting enzyme and risk of coronary artery disease. Mol Biol Rep 40, 4959–4965 (2013). https://doi.org/10.1007/s11033-013-2596-1

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