Molecular Biology Reports

, Volume 40, Issue 2, pp 1397–1405

Identification of genes differentially expressed in the roots of rubber tree (Hevea brasiliensis Muell. Arg.) in response to phosphorus deficiency

Authors

  • Peng He
    • Ministry of Agriculture Key Laboratory for Rubber BiologyRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences
    • Ministry of Agriculture Key Laboratory for Rubber BiologyRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences
  • Min Wu
    • Ministry of Agriculture Key Laboratory for Rubber BiologyRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences
  • Bingsun Wu
    • Ministry of Agriculture Key Laboratory for Rubber BiologyRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences
  • Jiashao Wei
    • Ministry of Agriculture Key Laboratory for Rubber BiologyRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences
  • Dapeng Wang
    • Ministry of Agriculture Key Laboratory for Rubber BiologyRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences
Article

DOI: 10.1007/s11033-012-2183-x

Cite this article as:
He, P., Qin, H., Wu, M. et al. Mol Biol Rep (2013) 40: 1397. doi:10.1007/s11033-012-2183-x
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Abstract

Phosphorus (P) is an essential macronutrient for plant growth and development. P deficiency could affect rubber tree productivity seriously, and understanding the mechanism responses of the rubber tree under the P deficiency will be helpful to improving rubber tree productivity. The molecular mechanism by which the rubber trees respond to a P-deficiency is a complex network involving many processes. To identify the genes differentially expressed in that response, we constructed subtractive suppression hybridization libraries for roots of plants growing under deficient or sufficient conditions. We identified 94 up-regulated genes from the forward library and 45 down-regulated from the reverse library. These differentially expressed genes were categorized into eight groups representing functions in metabolism, transcription, signal transduction, protein synthesis, transport, stress responses, photosynthesis, and development. We also performed quantitative real-time PCR to investigate the expression profiles of eight randomly selected clones. Our results provide useful information for further study of the molecular mechanism for adaptations to a P-deficiency in this species. Further characterization and functional analysis of these differentially expressed genes will help us improve its phosphorus utilization and overall productivity.

Keywords

Rubber treePhosphorus deficiencySubtractive suppression hybridizationDifferentially expressed gene

Copyright information

© Springer Science+Business Media Dordrecht 2012