Molecular Biology Reports

, Volume 40, Issue 1, pp 117–127

Cloning and characterization of two rice long-chain base kinase genes and their function in disease resistance and cell death

  • Huijuan Zhang
  • Li Li
  • Yongmei Yu
  • Jibo Mo
  • Lijun Sun
  • Bo Liu
  • Dayong Li
  • Fengming Song
Article

DOI: 10.1007/s11033-012-2040-y

Cite this article as:
Zhang, H., Li, L., Yu, Y. et al. Mol Biol Rep (2013) 40: 117. doi:10.1007/s11033-012-2040-y
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Abstract

Sphingolipid metabolites such as long-chain base 1-phosphates (LCBPs) have been shown to play an important role in plants; however, little is known about their function in plant disease resistance and programmed cell death (PCD). In the present study, we cloned and identified two rice long-chain base kinase (LCBK) genes (OsLCBK1 and OsLCBK2), which are involved in biosynthesis of LCBPs, and performed functional analysis in transgenic tobacco. Expression of OsLCBK1 and OsLCBK2 was induced in rice seedlings after treatments with defense signaling molecules and after infection by Magnaporthe grisea, the causal agent of blast disease. Transgenic tobacco plants overexpressing OsLCBK1 were generated and disease resistance assays indicate that the OsLCBK1-overexpressing plants showed enhanced disease resistance against Pseudmonassyringae pv. tabacci, the causal agent of wildfire disease, and tobacco mosaic virus. Expression levels of some defense-related genes were constitutively up-regulated and further induced after pathogen infection in the OsLCBK1-overexpressing plants. Treatment with fungal toxin fumonisin B1, an effective inducer of PCD in plants, resulted in reduced level of cell death in the OsLCBK1-overexpressing plants, as indicated by cell death staining, leakage of electrolyte and expression of hypersensitive response indicator genes. These data suggest that rice LCBKs, probably through regulation of endogenous LCBP level, play important roles in disease resistance response and PCD in plants.

Keywords

Rice (Oryza sativa L.)Long-chain base 1-phosphate (LCBP)Long-chain base kinase (LCBK)Transgenic tobaccoDisease resistanceProgrammed cell death

Abbreviations

ABA

Abscisic acid

ACC

1-Amino cyclopropane-1-carboxylic acid

BTH

Benzothidiazole

Cer

Ceramide

ET

Ethylene

FB1

Fumonisin B1

HR

Hypersensitive response

JA

Jasmonic acid

LCB

Long-chain base

LCBK

Long-chain base kinase

LCBP

Long-chain base 1-phosphate

PCD

Programed cell death

Pst

Pseudomonas syringae pv. tobacci

RT-PCR

Reverse transcriptase-polymerase chain reaction

S1P

Sphingosine-1-phosphate

SA

Salicylic acid

SphK

Sphingosine kinase

TMV

Tobacco mosaic virus

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Huijuan Zhang
    • 1
  • Li Li
    • 1
  • Yongmei Yu
    • 1
  • Jibo Mo
    • 1
  • Lijun Sun
    • 1
  • Bo Liu
    • 1
  • Dayong Li
    • 1
  • Fengming Song
    • 1
    • 2
  1. 1.State Key Laboratory for Rice Biology, Institute of BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Department of Plant Protection, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China