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Interactions of interleukin-12A and interleukin-12B polymorphisms on the risk of intracranial aneurysm

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Abstract

Several lines of evidence indicate that inflammatory processes play pivotal role in the development of intracranial aneurysm (IA). Recently, polymorphisms in the interleukin-12 (IL-12) gene were shown to be associated with immune-mediated inflammatory disease. The aim of this study was to investigate the interactions of IL-12A and IL-12B polymorphisms on the risk of IA in a Chinese population. A total of 422 individuals (including 164 patients with IA and 258 controls) were involved in the study. The polymorphisms (i.e., rs2243115 and rs568408 in IL-12A and rs3212227 in IL-12B) were genotyped by polymerase chain reaction–restriction fragment length polymorphism assay and DNA sequencing. We found an association of the AC/CC genotypes and C allele of IL-12B rs3212227 with an increased risk of IA, compared with the AA genotype and A allele (AC/CC vs. AA: OR = 2.09, 95 % CI: 1.29–3.38; C vs. A: OR = 1.45, 95 % CI: 1.10–1.91). Moreover, a significant gene interaction of IL-12A and IL-12B was evident on the risk of IA, and subjects carrying variant genotypes of IL-12B rs3212227 had an increased risk of IA. In the stratified analysis by gender, the IL-12B rs3212227 AC/CC genotypes had an increased risk of IA compared with the AA genotype in male patients (AC/CC vs. AA: OR = 4.63, 95 % CI: 1.92–11.16). These findings suggest that the IL-12A and IL-12B independently and jointly be involved in the susceptibility to IA.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30801317, 30901720), and Science & Technology Pillar Program of Sichuan Province (No. 2010SZ0122).

Conflict of interest

None of the authors has any potential financial conflict of interest related to this manuscript.

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Authors and Affiliations

  1. Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, People’s Republic of China

    Li-Juan Li, Lu-Shun Zhang, Yi Zhu, Wei-Bo Liang & Lin Zhang

  2. Department of Forensic Pathology, Henan University of Science and Technology, Luoyang, 471003, Henan, People’s Republic of China

    Xin-Min Pan

  3. Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, 610041, Sichuan, People’s Republic of China

    Xiutian Sima

  4. Department of Gastrointestinal and Pancreatic Surgery, Luoyang Central Hospital, Luoyang, 471009, Henan, People’s Republic of China

    Zhao-Hui Li

  5. Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children’s Health, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, People’s Republic of China

    Hong Sun & Lin-Bo Gao

  6. Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu, 610041, Sichuan, People’s Republic of China

    Lin-Bo Gao

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  1. Li-Juan Li
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  2. Xin-Min Pan
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Correspondence to Lin-Bo Gao or Lin Zhang.

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Li-Juan Li and Xin-Min Pan contributed equally to this work.

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Li, LJ., Pan, XM., Sima, X. et al. Interactions of interleukin-12A and interleukin-12B polymorphisms on the risk of intracranial aneurysm. Mol Biol Rep 39, 11217–11223 (2012). https://doi.org/10.1007/s11033-012-2031-z

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  • DOI: https://doi.org/10.1007/s11033-012-2031-z

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