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Prevalence of coagulation factor II G20210A and factor V G1691A Leiden polymorphisms in Chechans, a genetically isolated population in Jordan

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Abstract

Background Coagulation factor II G20210A and coagulation factor V (Leiden) G1691A single nucleotide polymorphisms (SNPs) are major inherited risk factors of venous thromboembolism. In view of the heterogeneity in their world distribution and lack of sufficient information about their distribution among Chechans, we addressed the prevalence of these SNPs in the Chechan population in Jordan, a genetically isolated population. Methods and Results factor II G20210A and factor V Leiden SNPs were analysed by polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP) method and Amplification refractory mutation detection system (ARMS) respectively in 120 random unrelated subjects from the Chechan population in Jordan. Among the subjects studied for factor II G20210A mutation there were three individuals carrying this mutation as heterozygous (one female and two male), giving a prevalence of 2.5 % and an allele frequency of 1.25 %. No homozygous factor II allele was found. Factor V Leiden G1691A mutation was detected as heterozygous in 22 of 120 of individuals (17 female and five male) indicating a prevalence of 18.3 % and allele frequency of 9.2 %. No homozygous allele was found. Conclusion Our results indicated that prevalence of factor II G20210A mutation in the Chechan population is similar to prevalence in Jordan and Caucasian populations (1–6 %) while the prevalence of factor V Leiden was higher in the Chechan population compared to Jordan and Caucasian populations (2–15 %).

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Acknowledgments

This study has been supported by the Hashemite University. We would like to thank Dr. M El Khateeb for giving us the opportunity to use the facilities at the National Center for Diabetes, Endocrinology and Genetics. We would also like to thank the Chechan community for their cooperation in this study.

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Authors and Affiliations

  1. Department of Biology and Biotechnology, Hashemite University, Zarqa, Jordan

    Rana Dajani

  2. National Center for Diabetes, Endocrinology and Genetics, Amman, Jordan

    Raja Fatahallah

  3. Faculty of Medicine, Hashemite University, Zarqa, Jordan

    Abdelrahman Dajani

  4. Laboratory of Human Embryology, Institute of Medical Biology, A*STAR, Singapore, Singapore

    Mohammad Al-Shboul

  5. Department of Community Medicine, Public Health and Family Medicine, Faculty of Medicine, Jordan University for Science and Technology, Irbid, Jordan

    Yousef Khader

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  1. Rana Dajani
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  2. Raja Fatahallah
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  3. Abdelrahman Dajani
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  4. Mohammad Al-Shboul
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  5. Yousef Khader
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Correspondence to Rana Dajani.

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Dajani, R., Fatahallah, R., Dajani, A. et al. Prevalence of coagulation factor II G20210A and factor V G1691A Leiden polymorphisms in Chechans, a genetically isolated population in Jordan. Mol Biol Rep 39, 9133–9138 (2012). https://doi.org/10.1007/s11033-012-1785-7

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  • DOI: https://doi.org/10.1007/s11033-012-1785-7

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