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Genetic effects of stearoyl-coenzyme A desaturase (SCD) polymorphism on milk production traits in the Chinese dairy population

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Abstract

Stearoyl-CoA desaturase (SCD) is a multifunctional complex enzyme important in the cellular biosynthesis of fatty acids. The present study was to investigate the association of the SCD gene with milk production traits in dairy cattle. Two single nucleotide polymorphisms (SNPs) (g.6926A>G and g.8646A>G) in introns 3 and 4, and three SNPs (g.10153A>G, g.10213T>C and g.10329C>T) in exon 5 were identified with pooled DNA sequencing and genotyped using matrix-assisted laser desorption/ionization time of flight mass spectrometry assay in 752 Chinese Holstein cows. Polymorphism g.10329C>T was predicted to result in an amino acid replacement from alanine to valine in the SCD protein. With a mixed animal model, the significant associations of the five SNPs with 305-day milk, fat and protein yields and protein percentage were determined. We further demonstrated cows with heterozygous genotypes (A/G or C/T) had highest 305 day milk yield, fat yield, protein yield and lowest protein percentage. Heterozygous cows with genotype AG at the g.6926A>G locus showed the greatest milk yield (P < 0.0001), fat yield (P < 0.0001) and protein yield (P < 0.0001) among other heterozygous genotypes at any of the loci. Dominance effects of all identified SNPs on milk, fat and protein yields and protein percentage were significant. Moreover, significant allele substitution effects at g.6926A>G locus on milk yield and at g.10213T>C on protein yield were observed. Five-locus haplotypes and strong linkage disequilibrium (D′ > 0.9) between the five SNPs were also observed. The results suggest that identified polymorphisms could be potential genetic markers to improve the production performance of Chinese Holstein.

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References

  1. Dekkers JCM (2004) Commercial application of marker-and gene-assisted selection in livestock: strategies and lessons. J Anim Sci 82(13 Electronic Supplement 1):E313

    PubMed  Google Scholar 

  2. Nott A, Meislin SH, Moore MJ (2003) A quantitative analysis of intron effects on mammalian gene expression. RNA 9(5):607–617

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Zan L, Zhang J, Liu X (2007) Association study on AGPAT6 intron3 polymorphism and milk performance of dairy cattle. Sci Agric Sin 40(7):1498–1503

    CAS  Google Scholar 

  4. Grisart B, Coppieters W, Farnir F, Karim L, Ford C, Berzi P, Cambisano N, Mni M, Reid S, Simon P (2002) Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Res 12(2):222–231

    Article  CAS  PubMed  Google Scholar 

  5. Hayes B, Goddard ME (2001) The distribution of the effects of genes affecting quantitative traits in livestock. Genet Sel Evol 33(3):209–230

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Khatib H, Monson RL, Schutzkus V, Kohl DM, Rosa GJM, Rutledge JJ (2008) Mutations in the STAT5A gene are associated with embryonic survival and milk composition in cattle. J Dairy Sci 91(2):784–793

    Article  CAS  PubMed  Google Scholar 

  7. Khatib H, Zaitoun I, Wiebelhaus-Finger J, Chang YM, Rosa GJM (2007) The association of bovine PPARGC1A and OPN genes with milk composition in two independent Holstein cattle populations. J Dairy Sci 90(6):2966–2970

    Article  CAS  PubMed  Google Scholar 

  8. Mele M, Conte G, Castiglioni B, Chessa S, Macciotta NP, Serra A, Buccioni A, Pagnacco G, Secchiari P (2007) Stearoyl-coenzyme A desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins. J Dairy Res 90(9):4458–4465. doi:10.3168/jds.2006-617

    Article  CAS  Google Scholar 

  9. Moioli B, Contarini G, Avalli A, Catillo G, Orru L, De Matteis G, Masoero G, Napolitano F (2007) Short communication: effect of stearoyl-coenzyme A desaturase polymorphism on fatty acid composition of milk. J Dairy Res 90(7):3553–3558. doi:10.3168/jds.2006-855

    Article  CAS  Google Scholar 

  10. Peterson DG, Kelsey JA, Bauman DE (2002) Analysis of variation in cis-9, trans-11 conjugated linoleic acid (CLA) in milk fat of dairy cows. J Dairy Res 85(9):2164–2172. doi:10.3168/jds.S0022-0302(02)74295-1

    Article  CAS  Google Scholar 

  11. Enoch HG, Catala A, Strittmatter P (1976) Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid. J Biol Chem 251(16):5095

    CAS  PubMed  Google Scholar 

  12. Shimakata T, Mihara K (1972) Reconstitution of hepatic microsomal stearoyl-coenzyme A desaturase system from solubilized components. J Biochem 72(5):1163–1174

    CAS  PubMed  Google Scholar 

  13. Strittmatter P, Spatz L, Corcoran D, Rogers MJ, Setlow B, Redline R (1974) Purification and properties of rat liver microsomal stearoyl coenzyme A desaturase. Proc Natl Acad Sci USA 71(11):4565

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Boichard D, Grohs C, Bourgeois F, Cerqueira F, Faugeras R, Neau A, Rupp R, Amigues Y, Boscher MY, Levéziel H (2003) Detection of genes influencing economic traits in three French dairy cattle breeds. Genet Sel Evol 35(1):77–102

    Article  PubMed  PubMed Central  Google Scholar 

  15. Druet T, Fritz S, Boichard D, Colleau JJ (2006) Estimation of genetic parameters for quantitative trait loci for dairy traits in the French Holstein population. J Dairy Res 89(10):4070–4076. doi:10.3168/jds.S0022-0302(06)72451-1

    Article  CAS  Google Scholar 

  16. Jiang Z, De S, Garcia MD, Griffin KB, Wu XL, Xiao Q, Michal JJ, Sharma BS, Jansen GB (2005) An independent confirmation of a quantitative trait locus for milk yield and composition traits on bovine chromosome 26. J Anim Breed Genet 122(4):281–284

    Article  CAS  PubMed  Google Scholar 

  17. Plante Y, Gibson JP, Nadesalingam J, Mehrabani-Yeganeh H, Lefebvre S, Vandervoort G, Jansen GB (2001) Detection of quantitative trait loci affecting milk production traits on 10 chromosomes in Holstein cattle. J Dairy Res 84(6):1516–1524. doi:10.3168/jds.S0022-0302(01)70185-3

    Article  CAS  Google Scholar 

  18. Viitala SM, Schulman NF, de Koning DJ, Elo K, Kinos R, Virta A, Virta J, Maki-Tanila A, Vilkki JH (2003) Quantitative trait loci affecting milk production traits in Finnish Ayrshire dairy cattle. J Dairy Res 86(5):1828–1836. doi:10.3168/jds.S0022-0302(03)73769-2

    Article  CAS  Google Scholar 

  19. Gautier M, Barcelona RR, Fritz S, Grohs C, Druet T, Boichard D, Eggen A, Meuwissen TH (2006) Fine mapping and physical characterization of two linked quantitative trait loci affecting milk fat yield in dairy cattle on BTA26. Genetics 172(1):425–436. doi:10.1534/genetics.105.046169

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Bernard L, Leroux C, Hayes H, Gautier M, Chilliard Y, Martin P (2001) Characterization of the caprine stearoyl-CoA desaturase gene and its mRNA showing an unusually long 3′-UTR sequence arising from a single exon* 1. Gene 281(1–2):53–61

    Article  CAS  PubMed  Google Scholar 

  21. Miyazaki M, Jacobson MJ, Man WC, Cohen P, Asilmaz E, Friedman JM, Ntambi JM (2003) Identification and characterization of murine SCD4, a novel heart-specific stearoyl-CoA desaturase isoform regulated by leptin and dietary factors. J Biol Chem 278(36):33904

    Article  CAS  PubMed  Google Scholar 

  22. Ward RJ, Travers MT, Richards SE, Vernon RG, Salter AM, Buttery PJ, Barber MC (1998) Stearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome1. Biochim Biophys Acta Lipids Lipid Metab 1391(2):145–156

    Article  CAS  Google Scholar 

  23. Zhang L, Ge L, Parimoo S, Stenn K, Prouty SM (1999) Human stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites. Biochem J 340(Pt 1):255–264

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Chung M, Ha S, Jeong S, Bok J, Cho K, Baik M, Choi Y (2000) Cloning and characterization of bovine stearoyl CoA desaturase1 cDNA from adipose tissues. Biosci Biotechnol Biochem 64(7):1526–1530

    Article  CAS  PubMed  Google Scholar 

  25. Taniguchi M, Utsugi T, Oyama K, Mannen H, Kobayashi M, Tanabe Y, Ogino A, Tsuji S (2004) Genotype of stearoyl-coA desaturase is associated with fatty acid composition in Japanese Black cattle. Mamm Genome 15(2):142–148

    Article  CAS  PubMed  Google Scholar 

  26. Lynch M, Walsh B (1997) Genetics and analysis of quantitative traits. Sinauer Associates, Inc., Sunderland

    Google Scholar 

  27. Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics, 4th edn. Longman Scientific and Technical, New York

    Google Scholar 

  28. Zhang S (2005) Association of genetic variation to healthfulness of beef. Lowa State University Animal Industry Report 2005. www.ans.iastate.edu/report/air/2005.pdf. Accessed 12 Dec 2009

  29. Bauman DE, Mather IH, Wall RJ, Lock AL (2006) Major advances associated with the biosynthesis of milk. J Dairy Sci 89(4):1235–1243

    Article  CAS  PubMed  Google Scholar 

  30. Soyeurt H, Dardenne P, Gillon A, Croquet C, Vanderick S, Mayeres P, Bertozzi C, Gengler N (2006) Variation in fatty acid contents of milk and milk fat within and across breeds. J Dairy Sci 89(12):4858–4865

    Article  CAS  PubMed  Google Scholar 

  31. Kgwatalala PM, Ibeagha-Awemu EM, Hayes JF, Zhao X (2007) Single nucleotide polymorphisms in the open reading frame of the stearoyl-CoA desaturase gene and resulting genetic variants in Canadian Holstein and Jersey cows. DNA Seq 18(5):357–362. doi:10.1080/10425170701291921

    Article  CAS  PubMed  Google Scholar 

  32. Schennink A, Heck JM, Bovenhuis H, Visker MH, van Valenberg HJ, van Arendonk JA (2008) Milk fatty acid unsaturation: genetic parameters and effects of stearoyl-CoA desaturase (SCD1) and acyl CoA: diacylglycerol acyltransferase 1 (DGAT1). J Dairy Res 91(5):2135–2143. doi:10.3168/jds.2007-0825

    Article  CAS  Google Scholar 

  33. Milanesi E, Nicoloso L, Crepaldi P (2008) Stearoyl CoA desaturase (SCD) gene polymorphisms in Italian cattle breeds. J Anim Breed Genet 125(1):63–67. doi:10.1111/j.1439-0388.2007.00697.x

    Article  CAS  PubMed  Google Scholar 

  34. Conte G, Mele M, Castiglioni B, Serra A, Viva M, Chessa S, Pagnacco G, Secchiari P (2006) Relationship between bovine SCD polymorphism locus and mammary gland desaturation activity. In: 8th world congress on genetics applied to livestock production, Belo Horizonte, Minas Gerais, Brazil, 2006. Instituto Prociancia, pp 22–37. URL: http://www.wcgalp8.org.br

  35. Macciotta NP, Mele M, Conte G, Serra A, Cassandro M, Dal Zotto R, Borlino AC, Pagnacco G, Secchiari P (2008) Association between a polymorphism at the stearoyl CoA desaturase locus and milk production traits in Italian Holsteins. J Dairy Res 91(8):3184–3189. doi:10.3168/jds.2007-0947

    Article  CAS  Google Scholar 

  36. Qiu H (2002) Modern dairy science. Agriculture Press of China, Beijing

    Google Scholar 

Download references

Acknowledgments

This work was supported by the Key Development of New Transgenic Breeds Program of China (2009ZX08009-156B) and National Natural Science Foundation of China (31072016), and National “948” Project (2011-G2A), The authors would also like to thank the Dairy Association of China for providing frozen semen samples, DHI data and pedigree information. The authors also would like to thank Dr. David Rheinheimer, Post-doctoral Researcher, Wuhan University, for his assistance with English expression and editing.

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Authors and Affiliations

  1. College of Animal Science and Technology, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, China Agricultural University, Beijing, 100193, China

    M. A. Alim, Y. P. Fan, X. P. Wu, Y. Xie, Y. Zhang, S. L. Zhang, D. X. Sun, Y. Zhang & Q. Zhang

  2. Beijing Dairy Cattle Center, Beijing, 100085, China

    L. Liu

  3. Beijing Sanyuan Lvhe Dairy Farming Cattle, Beijing, 102600, China

    G. Guo

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  1. M. A. Alim
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Correspondence to D. X. Sun.

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M. A. Alim and Y. P. Fan are the authors have contributed equally to this work.

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Alim, M.A., Fan, Y.P., Wu, X.P. et al. Genetic effects of stearoyl-coenzyme A desaturase (SCD) polymorphism on milk production traits in the Chinese dairy population. Mol Biol Rep 39, 8733–8740 (2012). https://doi.org/10.1007/s11033-012-1733-6

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