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Antioxidative potential of lactobacilli isolated from the gut of Indian people

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Abstract

Oxidative stress is one of the major causes of degenerative conditions occurring at cellular level with serious health implications. This study was aimed at investigating the antioxidative potentials of probiotic lactobacilli of Indian gut origin and their ability to augment antioxidant defense enzyme systems in the host cells under oxidative stress conditions. A total of 39 Lactobacillus cultures were assessed for their resistance against reactive oxygen species. Most of the cultures were moderately to strongly resistant towards 0.4 mM H2O2. The Lactobacillus isolate CH4 was the most H2O2 resistant culture with only 0.06 log cycle reduction. Majority of the cultures demonstrated high resistance towards hydroxyl ions and Lp21 was the most resistant with log count reduction of 0.20 fold only. Almost all the cultures were also quite resistant to superoxide anions. Lp21 also showed the highest superoxide dismutase content (0.8971 U). Amongst the 39 cultures, Lactobacillus spp. S3 showed the highest total antioxidative activity of 77.85 ± 0.13 % followed by Lp55 (56.1 ± 1.2 %) in terms of per cent inhibition of linolenic acid oxidation. Lp9 up-regulated the expression of superoxide dismutase 2 gene in HT-29 cells both at 0.1 mM (1.997 folds) and 1.0 mM H2O2 (2.058 folds) concentrations. In case of glutathione peroxidase-1, Lp9, Lp91 and Lp55 showed significant (P < 0.001) up-regulation in the gene expression to the level of 5.451, 8.706 and 10.083 folds, respectively when HT-29 was challenged with 0.1 mM H2O2. The expression of catalase gene was also significantly up-regulated by all the cultures at 0.1 mM H2O2 conditions. It can be concluded that the antioxidative efficacy of the putative probiotic lactobacilli varied considerably between species and strains and the potential strains can be explored as prospective antioxidants to manage oxidative stress induced diseases.

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Acknowledgments

We gratefully acknowledge the Director, National Dairy Research Institute (NDRI, Karnal, India) for providing facilities to carry out the study. The financial support received from National Dairy Research Institute (NDRI, India) and Indian Council of Agricultural Research (ICAR, India) in terms of providing fellowship to the first author of the paper to carry out her Master’s programme is greatly appreciated.

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Authors and Affiliations

  1. Molecular Biology Unit, Department of Dairy Microbiology, National Dairy Research Institute, Karnal, 132001, Haryana, India

    Anju A. Achuthan, Anupama Madathil, Harsh Panwar, Virender Kumar Batish & Sunita Grover

  2. Department of Food Engineering and Technology, Tezpur University, Napaam, 784028, Assam, India

    Raj Kumar Duary

  3. Lab.3, Molecular Biology Unit, National Centre for Cell Science, Pune University Campus, Pune, 411007, Maharashtra, India

    Himanshu Kumar

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  1. Anju A. Achuthan
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  2. Raj Kumar Duary
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  3. Anupama Madathil
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Correspondence to Sunita Grover.

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Achuthan, A.A., Duary, R.K., Madathil, A. et al. Antioxidative potential of lactobacilli isolated from the gut of Indian people. Mol Biol Rep 39, 7887–7897 (2012). https://doi.org/10.1007/s11033-012-1633-9

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