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Molecular characterization of a KIF3B-like kinesin gene in the testis of Octopus tankahkeei (Cephalopoda, Octopus)

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Abstract

KIF3B is known for maintaining and assembling cilia and flagellum. To date, the function of KIF3B and its relationship with KIF3A during spermiogenesis in the cephalopod Octopus tankahkeei remains unknown. In the present study, we characterized a gene encoding a homologue of rat KIF3B in the O. tankahkeei testis and examined its temporal and spatial expression pattern during spermiogenesis. The cDNA of KIF3B was obtained with degenerate and RACE PCR and the distribution pattern of ot-kif3b were observed with RT-PCR. The morphological development during spermiogenesis was illustrated by histological and transmission electron microscopy and mRNA expression of ot-kif3b was observed by in situ hybridization. The 2,365 nucleotides cDNA consisted of a 102 bp 5′ untranslated region (UTR), a 2,208 bp open reading frame (ORF) encoding a protein of 736 amino acids, and a 55 bp 3′ UTR. Multiple alignments revealed that the putative Ot-KIF3B shared 68, 68, 69, 68, and 67% identity with that of Homo sapiens, Mus musculus, Gallus gallus, Danio rerio, and Xenopus laevis, respectively, along with high identities with Ot-KIF3A in fundamental structures. Ot-kif3b transcripts appeared gradually in early spermatids, increased in intermediate spermatids and maximized in drastically remodeled and final spermatids. The kif3b gene is identified and its expression pattern is demonstrated for the first time in O. tankahkeei. Compared to ot-kif3a reported by our laboratory before, our data suggested that the putative heterodimeric motor proteins Ot-KIF3A/B may be involved in intraspermatic transport and might contribute to structural changes during spermiogenesis.

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Acknowledgments

We would like to thank all the members of the Sperm Laboratory at Zhejiang University. We are also grateful to Mr. Jia-Qiang Yan and other members in Professor Zhu Jun-Quan’s laboratory at Ningbo University for materials preparation. This project was supported in part by: (1) Zhejiang Provincial Natural Science Foundation of China (Grant No Z307536 and Y2100296); (2) National Natural Science Foundation of China, Grant number: No. 31072198 and 40776079; (3) K. C. Wong Magna Fund in Ningbo University; (4) the Scientific Research Foundation of Graduate School of Ningbo University (Grant No NG09JLA013).

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Authors and Affiliations

  1. Faculty of Life Science and Bioengineering, Ningbo University, 315211, Zhejiang, People’s Republic of China

    Ran Dang, Jun-Quan Zhu & Wei Wang

  2. The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Ran Dang, Wei Wang, Hong Zhou & Wan-Xi Yang

  3. The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, People’s Republic of China

    Fu-Qing Tan

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  1. Ran Dang
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  2. Jun-Quan Zhu
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  3. Fu-Qing Tan
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  4. Wei Wang
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Correspondence to Wan-Xi Yang.

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Ran Dang, Jun-Quan Zhu, and Fu-Qing Tan contributed equally to this work.

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Dang, R., Zhu, JQ., Tan, FQ. et al. Molecular characterization of a KIF3B-like kinesin gene in the testis of Octopus tankahkeei (Cephalopoda, Octopus). Mol Biol Rep 39, 5589–5598 (2012). https://doi.org/10.1007/s11033-011-1363-4

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