Skip to main content
SpringerLink
Log in

Association of FATP1 gene polymorphisms with chicken carcass traits in Chinese meat-type quality chicken populations

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

In this study, we aimed to detect the single nucleotide polymorphisms (SNPs) of the chicken FATP1 gene and discern the potential association between FATP1 SNPs and chicken carcass traits. A total of 620 meat-type quality chickens from six commercial pure lines (S01, S02, S03, S05, S06 and D99) and two cross lines (S05 × S01 and S06 × S01) were screened by using the single-strand conformational polymorphism analysis (SSCP) and DNA sequencing. Five SNPs [g.49360G > A, g.48195G > A, g.46847A > G, g.46818A > G, and g.46555A > G] were identified in chicken FATP1 gene. SNP g.46818 A > G was a rare variant and was not considered in the subsequent analysis. Sixteen haplotypes were reconstructed on the basis of the other four SNPs. The linear regression model analysis indicated that there were significant associations of certain diplotypes with part of carcass traits, such as live weight (LW), carcass weight (CW), and semi-eviscerated weight (SEW) (P < 0.05). In particular, diplotype H2H4 had a negative effect on LW, CW, SEW, and abdominal fat weight (AW); diplotype H6H10 had the highest reducing effect on subcutaneous fat thickness (SFT). Our results suggested that FATP1gene polymorphisms were associated with chicken carcass traits or was linked with the major gene. The SNPs in this gene may be utilized as potential markers for marker-assisted selection (MAS) during chicken breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Chen GH, Wu XS, Hou SS (1999) Comparison on relative muscle fatty acids content in Chinese native chickens. Chin J Animal Sci 35:27–28 (in Chinese)

    Google Scholar 

  2. Huang ZG, Xie Z (2004) The development of animal adipose related genes. Animal Husb Vet Med 4:41–43 (in Chinese)

    Google Scholar 

  3. Zhou SL, Stump D, Sorrentino D (1992) Adipocyte differentiation of 3T3–L1 cells involves augmented expression of a 43-kDa plasma membrane fatty acid-binding protein. J Biol Chem 268:14456–14461

    Google Scholar 

  4. Griffin HD, Guo K, Windsor D (1992) Adipose tissue lipoge and fat deposition in leaner broiler chickens. J Nutr 122:363–368

    CAS  PubMed  Google Scholar 

  5. Stahl A, Gimeno RE, Tartaglia LA, Lodish HF (2001) Fatty acid transport proteins: a current view of a growing family. Trends Endocrinol Metab 12:266–273

    Article  CAS  PubMed  Google Scholar 

  6. Hirsch D, Stahl A, Lodish HF (1998) A family of fatty acid transporters conserved from mycobacterium to man. Proc Natl Acad Sci USA 95:8625–8629

    Article  CAS  PubMed  Google Scholar 

  7. Pohl J, Ring A, Hermann T, Stremmel W (2004) Role of FATP in parenchymal cell fatty acid uptake. Biochim Biophys Acta 1686:1–6

    CAS  PubMed  Google Scholar 

  8. Schaffer JE, Lodish HF (1994) Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein. Cell 79:427–436

    Article  CAS  PubMed  Google Scholar 

  9. Doege H, Stahl A (2006) Protein-mediated fatty acid uptake: novel insights from in vivo models. Physiology (Bethesda) 21:259–268

    CAS  Google Scholar 

  10. Meirhaeghe A, Martin G, Nemoto M, Deeb S, Cottel D, Auwerx J, Amouyel P, Helbecque N (2000) Intronic polymorphism in the fatty acid transport protein 1 gene is associated with increased plasma triglyceride levels in a French population. Arterioscler Thromb Vasc Biol 20:1330–1334 Erratum in: Arterioscler Thromb Vasc Biol 2005; 25(10):e139

    CAS  PubMed  Google Scholar 

  11. Meirhaeghe A, Cottel D, Amouyel P, Dallongeville J (2005) Lack of association between certain candidate gene polymorphisms and the metabolic syndrome. Mol Genet Metab 86:293–299

    Article  CAS  PubMed  Google Scholar 

  12. Binnert C, Koistinen HA, Martin G, Andreelli F, Ebeling P, Koivisto VA, Laville M, Auwerx J, Vidal H (2000) Fatty acid transport protein-1 mRNA expression in skeletal muscle and in adipose tissue in humans. Am J Physiol 279:1072–1079

    Google Scholar 

  13. Zhang JQ, Chen H, Sun ZJ, Liu XL, Qiang-Ba YZ, Gu YL (2010) Flesh color association with polymorphism of the Tyrosinase gene in different Chinese chicken breeds. Mol Biol Rep 37(1):165–169

    Article  CAS  PubMed  Google Scholar 

  14. Fang M, Nie Q, Luo C, Zhang D, Zhang X (2010) Associations of GHSR gene polymorphisms with chicken growth and carcass traits. Mol Biol Rep 37(1):423–428

    Article  CAS  PubMed  Google Scholar 

  15. Zhang ZR, Liu YP, Yao YG, Jiang XS, Du HR, Zhu Q (2009) Identification and association of the single nucleotide polymorphisms in calpain 3 (CANP3) gene with carcass traits in chickens. BMC Genet 10:10. doi:10.1186/1471-2156-10-10

    Article  CAS  PubMed  Google Scholar 

  16. Zhou Y, Liu YP, Jiang XS, Du HR, Li XC, Zhu Q (2010) Polymorphism of chicken Myocyte-specific enhancer-binding factor 2A gene and its association with chicken carcass traits. Mol Biol Rep 37:587–594

    Article  CAS  PubMed  Google Scholar 

  17. Stephens M, Smith N, Donnelly P (2001) A new statistical method for haplotype reconstruction from population data. Am J Hum Genet 68:978–989

    Article  CAS  PubMed  Google Scholar 

  18. Gertow K, Skoglund-Andersson C, Eriksson P, Boquist S, Orth-Gomer K, Schenck-Gustafsson K, Hamsten A, Fisher RM (2003) A common polymorphism in the fatty acid transport protein-1 gene associated with elevated post-prandial lipaemia and alterations in LDL particle size distribution. Atherosclerosis 167:265–273

    Article  CAS  PubMed  Google Scholar 

  19. Daly MJ, Rioux JD, Schaffner SF, Hudson TJ, Lander ES (2001) High-resolution haplotype structure in the human genome. Nat Genet 2:229–232

    Article  Google Scholar 

  20. Huang QQ, Fu YX, Boerwinkle E (2003) Comparison of strategies for selecting single nucleotide polymorphisms for case/control association studies. Hum Genet 113:253–257

    Article  CAS  PubMed  Google Scholar 

  21. Stephens JC, Schneider JA, Tanguay DA, Choi J, Acharya T, Stanley SE, Jiang RH, Messer CJ, Chew A, Han JH, Duan JC, Carr JL, Lee MS, Koshy B, Kumar AM, Zhang G, Newell WR, Windemuth A, Xu CB, Kalbfleisch TS, Shaner SL, Arnold K, Schulz V, Drysdale CM, Nandabalan K, Judson RS, Ruano G, Vovis GF (2001) Haplotype variations and linkage disequilibrium in 313 human genes. Science 293:489–493

    Article  CAS  PubMed  Google Scholar 

  22. Zhang WH, Collins A, Morton NE (2004) Does haplotype diversity predict power for association mapping of disease susceptibility. Hum Genet 115:157–160

    Article  PubMed  Google Scholar 

  23. Cardon LR, Abecasis GR (2003) Using haplotype blocks to map human complex trait loci. Trends Genet 19:135–140

    Article  CAS  PubMed  Google Scholar 

  24. Croucher PJ, Mascheretti S, Hampe J, Huse K, Frenzel H, Stoll M, Lu T, Nikolaus S, Yang SK, Krawczak M, Kim WH, Schreiber S (2003) Haplotype structure and association to Crohn’s disease of CARD15 mutations in two ethnically divergent populations. Eur J Hum Genet 11:6–16

    Article  CAS  PubMed  Google Scholar 

  25. Lin DY (2004) Haplotype-based association analysis in cohort studies of unrelated individuals. Genet Epidemiol 26:255–264

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank Hua-Rui Du and Xiao-Song Jiang for sample collecting. This work was financially supported by the National 863 Project of China (2008AA101001), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), and Yunnan Province (2008CD175 and 2009CI119).

Author information

Authors and Affiliations

  1. College of Animal Science and Technology, Sichuan Agricultural University, Xinkang Road 46#, Ya’an, 625014, Sichuan, People’s Republic of China

    Yan Wang, Qing Zhu, Xiao-Ling Zhao & Yi-Ping Liu

  2. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, 650223, Kunming, Yunnan, China

    Yong-Gang Yao

Authors
  1. Yan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qing Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiao-Ling Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yong-Gang Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yi-Ping Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yong-Gang Yao or Yi-Ping Liu.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 121 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Y., Zhu, Q., Zhao, XL. et al. Association of FATP1 gene polymorphisms with chicken carcass traits in Chinese meat-type quality chicken populations. Mol Biol Rep 37, 3683–3690 (2010). https://doi.org/10.1007/s11033-010-0020-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-010-0020-7

Keywords

Search

Navigation