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Sevoflurane postconditioning protects isolated rat hearts against ischemia-reperfusion injury: the role of radical oxygen species, extracellular signal-related kinases 1/2 and mitochondrial permeability transition pore

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Abstract

The roles of reactive oxygen species (ROS), extracellular signal-regulated kinase 1/2 (ERK 1/2) and mitochondrial permeability transition pore (mPTP) in sevoflurane postconditioning induced cardioprotection against ischemia-reperfusion injury in Langendorff rat hearts were investigated. When compared with the unprotected hearts subjected to 30 min of ischemia followed by 1 h of reperfusion, exposure of 3% sevoflurane during the first 15 min of reperfusion significantly improved functional recovery, decreased infarct size, reduced lactate dehydrogenase and creatine kinase-MB release, and reduced myocardial malondialdehyde production. However, these protective effects were abolished in the presence of either ROS scavenger N-acetylcysteine or ERK 1/2 inhibitor PD98059, and accompanied by prevention of ERK 1/2 phosphorylation and elimination of inhibitory effect on mPTP opening. These findings suggested that sevoflurane postconditioning protected isolated rat hearts against ischemia-reperfusion injury via the recruitment of the ROS-ERK 1/2-mPTP signaling cascade.

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Acknowledgments

The authors thank Miss Ding-Hua Liu for her excellent technical support.

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Authors and Affiliations

  1. Department of Anesthesiology, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 100037, Beijing, China

    Yun-Tai Yao, Li-Huan Li, Lei Chen & Wei-Peng Wang

  2. Department of Cardiovascular Surgery and Cardiovascular Institute, General Hospital of the People’s Liberation Army, 100853, Beijing, China

    Li-Bing Li & Chang-Qing Gao

  3. No. 167 Beilishi Road, 100037, Xicheng District, Beijing, China

    Li-Huan Li

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  1. Yun-Tai Yao
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Correspondence to Li-Huan Li.

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Yao, YT., Li, LH., Chen, L. et al. Sevoflurane postconditioning protects isolated rat hearts against ischemia-reperfusion injury: the role of radical oxygen species, extracellular signal-related kinases 1/2 and mitochondrial permeability transition pore. Mol Biol Rep 37, 2439–2446 (2010). https://doi.org/10.1007/s11033-009-9755-4

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