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Purification and characterization of the cold-active alkaline protease from marine cold-adaptive Penicillium chrysogenum FS010

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Abstract

An extracellular cold-active alkaline serine protease from Penicillium chrysogenum FS010 has been purified. The purification procedure involved: ammonium sulfate precipitation, DEAE ion-exchange chromatography and sephadex G-100 gel chromatography. SDS–PAGE of the purified enzyme indicated a molecular weight of 41,000 ± 1,000 Da. The protease is stable in a pH range of 7.0–9.0 and has a maximum activity at pH 9.0. Compared with other industrial proteases, the enzyme shows a high hydrolytic activities at lower temperatures and a high sensitivity at a temperature over 50°C. The isoelectric point of the enzyme is approximate to 6.0. Enzymatic activity is enhanced by the addition of divalent cations such as Mg2+ and Ca2+ and inhibited by addition of Cu2+and Co2+. PMSF and DFP are its specific inhibitors. The application of the cold-active alkaline protease is extremely extensive, and widely used in detergents, feed, food, leather and many other industries.

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Acknowledgments

The authors thank Ren Hongwei and Sun Caiyun (The State Key Laboratory of Microbial Technology, Shandong University, Jinan, and People’s Republic of China) for experimental assistances.

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, People’s Republic of China

    Hui-Yuan Zhu & Tian-hong Wang

  2. College of Food and Biological Engineering, Shandong Institute of Light Industry, 250353, Jinan, Shandong, People’s Republic of China

    Hui-Yuan Zhu, Yong Tian & Yun-Hua Hou

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  1. Hui-Yuan Zhu
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  2. Yong Tian
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  3. Yun-Hua Hou
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  4. Tian-hong Wang
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Correspondence to Tian-hong Wang.

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Zhu, HY., Tian, Y., Hou, YH. et al. Purification and characterization of the cold-active alkaline protease from marine cold-adaptive Penicillium chrysogenum FS010. Mol Biol Rep 36, 2169–2174 (2009). https://doi.org/10.1007/s11033-008-9431-0

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  • DOI: https://doi.org/10.1007/s11033-008-9431-0

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