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Effect of Lr34/Yr18 on agronomic and quality traits in a spring wheat mapping population and implications for breeding

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Abstract

The locus Lr34/Yr18 plays an important role in conferring resistance to a number of fungal diseases and is thus an important component of global wheat breeding efforts. We investigated the differences in disease response and agronomic traits of the ‘CDC Teal’ × ‘CDC Go’ spring wheat population of 187 recombinant inbred lines (RILs) in relation to the presence/absence of the rust resistance gene Lr34/Yr18. Lines carrying the resistant allele of Lr34/Yr18 were taller, matured earlier, and yielded less grain with lower test weights than lines without Lr34/Yr18. Lines with or without the resistant allele of Lr34/Yr18 did not differ for grain protein content, SDS sedimentation volume, and for resistance to leaf spotting and common bunt. Lines with Lr34/Yr18 exhibited lower leaf and stripe rust infection than lines without it. We selected superior lines from the population based on high yield, protein content, SDS sedimentation, and the presence of the resistant allele of Lr34/Yr18 and grew them with continued selection in replicated yield trials over nine site-years. We attempted to combine Lr34/Yr18 with high yield, protein content, and SDS sedimentation suitable for the Canadian western red spring wheat class. Our results suggested that the population size we used was not large enough to obtain recombinants with high yield potential, high grain protein, and acceptable quality attributes. Moreover, selection for Lr34/Yr18 resulted in the elimination of lines with high yield potential. We therefore suggest using a population size of at least 310 to increase the potential of pooling Lr34/Yr18 with high grain yield and desirable agronomic and end-use quality attributes.

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Acknowledgments

The authors would like to acknowledge and thank Klaus Strenzke, Glen Hawkins, Fabiana Dias, Alex Pswarayi, Joe Back, Ivan Adamyk, Henry Song, Graham Collier, Mohammad Asif, and Neshat Pazooki for technical assistance. This research was supported by grants to the University of Alberta wheat breeding program from the Alberta Crop Industry Development Fund, Western Grains Research Foundation and Alberta Wheat Commission (Grants # 2014F013R and 2014F014R), and Western Grains Research Foundation Endowment Fund (Grant #U1313611671S5-E) to D. Spaner. We would also like to acknowledge Agriculture and Agri-Food Canada (Grant #140915) and Genome Canada and Genome Alberta (Grant #CTAG). The first author received a scholarship from China Scholarship Council.

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Authors and Affiliations

  1. Agricultural, Food and Nutritional Science, 4-10 Ag/Forestry Centre, University of Alberta, Edmonton, AB, T6G 2P5, Canada

    Hua Chen, Muhammad Iqbal, Rong-Cai Yang & Dean Spaner

  2. National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Centre, Islamabad, 45500, Pakistan

    Muhammad Iqbal

  3. Alberta Agriculture and Forestry, #307, J.G. O’Donoghue Building, 7000-113 Street, Edmonton, AB, T6H 5T6, Canada

    Rong-Cai Yang

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  1. Hua Chen
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  2. Muhammad Iqbal
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  3. Rong-Cai Yang
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  4. Dean Spaner
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Correspondence to Muhammad Iqbal.

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Chen, H., Iqbal, M., Yang, RC. et al. Effect of Lr34/Yr18 on agronomic and quality traits in a spring wheat mapping population and implications for breeding. Mol Breeding 36, 53 (2016). https://doi.org/10.1007/s11032-016-0478-7

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