Abstract
The papaya ring spot virus (PRSV) causes significant fruit yield loss in cucurbit crops. Understanding of the inheritance and molecular mapping of PRSV resistance will facilitate development of resistant varieties to control this disease. In the present study, an F2 population was developed from the cross between susceptible ‘65G’ and resistant ‘02245’ cucumber inbred lines. Genetic analysis of PRSV resistance in 144 F2:3-derived F3 families showed that resistance is controlled by a single recessive gene which was designated as prsv 02245. Simple sequence repeat (SSR) markers were employed in polymorphism screening between PRSV-susceptible and resistant DNA pools. The PRSV resistance gene, prsv 02245, was mapped on chromosome 6 that was flanked by two SSR markers, SSR11-177 and SSR11-1, which was 1.1 and 2.9 cM away from the prsv 02245 locus, respectively. The physical distance between the two markers was approximately 600 kb. The accuracy rate of marker-assisted selection of PRSV resistance among 35 cucumber lines using the marker, SSR11-177 was more than 80 %. Results from this study provide a valuable tool for fine mapping, gene cloning, and marker-assisted breeding for PRSV resistance in cucumber.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amano M, Mochizuki A, Kawagoe Y, Iwahori K, Niwa K, Svoboda J, Maeda T, Imura Y (2013) High-resolution mapping of zym, a recessive gene for Zucchini yellow mosaic virus resistance in cucumber. Theor Appl Genet 126:2983–2993
Attasart P, Charoensilp G, Kertbundit S, Panyim S, Juricek M (2002) Nucleotide sequence of a Thai isolate of Papaya ringspot virus type W. Acta Virol 46:241–246
Bateson MF, Henderson J, Chaleeprom W, Gibbs AJ, Dale JL (1994) Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia). J Gen Virol 75:3547–3553
Bateson MF, Lines RE, Revill P, Chaleeprom W, Ha CV, Gibbs AJ, Dale JL (2002) On the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus. J Gen Virol 83:2575–2585
Gonsalves D (1998) Control of papaya ringspot virus in papaya: a case study. Annu Rev Phytopathol 36:415–437
Huang SW, Li RQ, Zhang ZH, Li L, Gu XF, Fan W, Lucas WJ, Wang XW, Xie BY, Ni PX, Ren YY, Zhu HM, Li J, Lin K, Jin WW, Fei ZJ, Li GC, Staub J, Kilian A, van der Vossen EAG, Wu Y, Guo J, He J, Jia ZJ, Ren Y, Tian G, Lu Y, Ruan J, Qian WB, Wang MW, Huang QF, Li B, Xuan ZL, Cao J, San A, Wu ZG, Zhang JB, Cai QL, Bai YQ, Zhao BW, Han YH, Li Y, Li XF, Wang SH, Shi QX, Liu SQ, Cho WK, Kim JY, Xu Y, Heller-Uszynska K, Miao H, Cheng ZC, Zhang SP, Wu J, Yang YH, Kang HX, Li M, Liang HQ, Ren XL, Shi ZB, Wen M, Jian M, Yang HL, Zhang GJ, Yang ZT, Chen R, Liu SF, Li JW, Ma LJ, Liu H, Zhou Y, Zhao J, Fang XD, Li GQ, Fang L, Li YR, Liu DY, Zheng HK, Zhang Y, Qin N, Li Z, Yang GH, Yang S, Bolund L, Kristiansen K, Zheng HC, Li SC, Zhang XQ, Yang HM, Wang J, Sun RF, Zhang BX, Jiang SZ, Wang J, Du YC, Li SG (2009) The genome of the cucumber, Cucumis sativus L. Nat Genet 41:1275–1281
Jensen DD (1949) Papaya virus diseases with special reference to papaya ringspot. Phytopathology 39:191–211
Kabelka E (1996) Allelic characterization and inheritance of potyvirus resistance genes in cucumber. Dissertation, East Lansing, Michigan: Michigan State University
Kabelka E, Grumet R (1997) Inheritance of resistance to the Moroccan watermelon mosaic virus in the cucumber line TMG-1 and cosegregation with zucchini yellow mosaic virus resistance. Euphytica 95:237–242
Kosaka Y, Song RB, Kobori T, Shiomi H, Yasuhara H, Kataoka M (2006) Effectiveness of an attenuated zucchini yellow mosaic virus isolate for cross-protecting cucumber. Plant Dis 90:67–72
Maule AJ, Caranta C, Boulton MI (2007) Sources of natural resistance to plant viruses: status and prospects. Mol Plant Pathol 8:223–231
Meyer JDF, Deleu W, Garcia-Mas J, Havey MJ (2008) Construction of a fosmid library of cucumber (Cucumis sativus) and comparative analyses of the eIF4E and eIF(iso)4E regions from cucumber and melon (Cucumis melo). Mol Genet Genomics 279:473–480
Ministry of Agriculture of the People’s Republic of China (2010) Rules for evaluation of cucumber for resistance to diseases. Part 7: rule for evaluation of cucumber for resistance to cucumber mosaic virus (NY/T 1857.7—2010 Beijing). (In Chinese)
Park YH, Sensoy S, Wye C, Antonise R, Peleman J, Havey MJ (2000) A genetic map of cucumber composed of RAPDs, RFLPs, AFLPs, and loci conditioning resistance to papaya ringspot and zucchini yellow mosaic viruses. Genome 43:1003–1010
Provvidenti R (1985) Source of resistance to virus in two accessions of Cucumis sativus. Cucurbit Genet Coop Rep 8:12
Purciful DE, Edwardson JR, Hiebert E, Consalves D (1984) Papaya ringspot virus. CMI/AAB Descr Plant Viruses 292:8
Qi JJ, Liu X, Shen D, Miao H, Xie BY, Li XX, Zeng P, Wang SH, Shang Y, Gu XF, Du YC, Li Y, Lin T, Yuan JH, Yang XY, Chen JF, Chen HM, Xiong XY, Huang K, Fei ZJ, Mao LY, Tian L, Städler T, Renner SS, Kamoun S, Lucas W, Zhang ZH, Huang SW (2013) A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity. Nat Genet 45:1510–1515
Ren Y, Zhang ZH, Liu JH, Staub JE, Han YH, Cheng ZH, Li XF, Miao H, Kang HX, Xie BY, Gu XF, Wang XW, Du YC, Jin WW, Huang SW (2009) An integrated genetic and cytogenetic map of the cucumber genome. PLoS ONE 4:e5795
Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY, Hunt MD (1996) Systemic acquired resistance. Plant Cell 8:1809–1819
Tomlinson JA (1987) Epidemiology and control of virus diseases of vegetables. Ann Appl Biol 110:661–681
Van Loon LC, Van Kammen A (1970) Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’ II. Changes in protein constitution after infection with tobacco mosaic virus. Virology 40:199–211
Wai T, Grumet R (1995) Inheritance of resistance to the watermelon strain of Papaya ringspot virus in the cucumber line TMG-1. HortScience 30:338–340
Wai T, Staub JE, Kabelka E, Grumet R (1997) Linkage analysis of potyvirus resistance alleles in cucumber. J Hered 88:454–458
Wang YJ, Provvidenti R, Robinson RW (1984) Inheritance of resistance in cucumber to Watermelon mosaic virus 1. HortScience 19:587–588
Wang HJ, Wu Y, Gu W, Sun XD, Qin ZW (2006) Extraction of DNA from cucumber by improved CTAB method. Heilongjiang Agric Sci 5:124–125 (in Chinese)
Zhang HY, Miao AJ, Zhang F, Wang YJ, Xu Y (2005) Genetic analysis of four major cucumber diseases. Acta Agric Boreali-Sin 20:100–103 (in Chinese)
Zhang SP, Miao H, Gu XF, Yang YH, Xie BY, Wang XW, Huang SW, Du YC, Sun RF (2010) Genetic mapping of the scab resistance gene in cucumber. J Am Soc Hort Sci 135:53–58
Zhang WW, Pan JS, He HL, Zhang C, Li Z, Zhao JL, Yuan G, Yuan XJ, Zhu LH, Huang SW, Cai R (2012) Construction of a high density integrated genetic map for cucumber (Cucumis sativus L.). Theor Appl Genet 124:249–259
Acknowledgments
This work was supported by the National High Technology Research and Development Program of China (863 Program, No. 2012AA100103); the earmarked fund for Modern Agro-industry Technology Research System (CARS-25) and the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China. The authors would like to thank Dr. Graham Collins, formerly of the University of Adelide, South Australia for proofreading.
Author information
Authors and Affiliations
Corresponding author
Additional information
Guili Tian and Yuhong Yang have contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Tian, G., Yang, Y., Zhang, S. et al. Genetic analysis and gene mapping of papaya ring spot virus resistance in cucumber. Mol Breeding 35, 110 (2015). https://doi.org/10.1007/s11032-015-0279-4
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11032-015-0279-4