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Genetic mapping of polygenic scab (Venturia pirina) resistance in an interspecific pear family

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Abstract

The need for chemical control of scab (Venturia pirina, V. nashicola) is the main barrier preventing sustainable pear production. As cultivars with durable resistance are not available yet commercially, the development of molecular markers for early selection is desirable to enhance the efficiency of breeding such cultivars. Interspecific pear progeny PEAR1 × PEAR2 derived from European (Pyrus communis) and Asian (P. pyrifolia and P. ussuriensis) pears was infected with three single-spore isolates of V. pirina using the droplet inoculation technique. Illumina Infinium® HD Assay technology was employed to genotype the progeny with single nucleotide polymorphism markers for map construction. With one linkage group missing in each parent, the parental maps covered 17 linkage groups in total, 1,132.3 and 1,136.8 centimorgan for the female and male parents, respectively. Resistance mapping resulted in the identification of seven quantitative trait loci (QTLs) by Kruskal–Wallis analysis. Parent PEAR1 contributed a QTL on linkage group (LG) 17 that was effective against all three scab isolates, while PEAR2 contributed one on LG7 that was effective against two isolates. The other five QTLs, on LG2 and LG5 of PEAR2, and LG7 and LG10 of PEAR1, displayed differential interactions, with each QTL being paired with a single incompatible isolate. Additive effects of combined resistance loci displayed a higher level of resistance than single loci, and the role of nonhost resistance is discussed.

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Acknowledgments

We thank Dianne Hyndman and Rosemary Rickman (AgResearch Invermay, New Zealand) for providing the Illumina genotyping service. We thank Dr Paul Johnston (PFR) for critical comments on the manuscript.

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Authors and Affiliations

  1. National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA), 121 Byeongnyugil, Geumcheon-Myeon, Naju, 520-821, Korea

    Kyungho Won, Yoon Kyeong Kim, Jang Hoon Song, Sam Seog Kang & Han Chan Lee

  2. The New Zealand Institute for Plant and Food Research Limited (Plant and Food Research), Private Bag 1401, Havelock North, 4157, New Zealand

    Héloïse Bastiaanse, Gagandeep Singla & Vincent G. M. Bus

  3. Plant and Food Research, Private Bag 11600, Palmerston North, 4442, New Zealand

    Susan E. Gardiner & David Chagné

  4. National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA), 475 Imok-dong, Jangan-gu, Suwon, 440-706, Korea

    Kang Hee Cho

  5. Plant and Food Research, 55 Old Mill Rd, RD 3, Motueka, 7198, New Zealand

    Lester Brewer

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  1. Kyungho Won
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  2. Héloïse Bastiaanse
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  3. Yoon Kyeong Kim
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  12. Vincent G. M. Bus
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Correspondence to Vincent G. M. Bus.

Electronic supplementary material

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11032_2014_172_MOESM1_ESM.docx

Supplemental material 1. The pedigrees of interspecific pear parents PEAR1 and PEAR2 used to map quantitative trait loci for scab resistance in this study. (DOCX 12 kb)

11032_2014_172_MOESM2_ESM.pptx

Supplemental material 2. Putative scab resistance QTLs on SNP maps of interspecific hybrid pears. Numbers on the left side indicate the distance in centimorgans (cM) from the top of each chromosome, and marker names (registered in NCBI) are presented on the right side. The marker most strongly associated with each of the QTLs is identified by their bold, red lettering, with the one broad-spectrum QTL also in italics. See Table 3 for the differential interactions between the Venturia pirina isolates and scab resistance QTLs identified by their most significant SNP marker. (PPTX 159 kb)

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Won, K., Bastiaanse, H., Kim, Y.K. et al. Genetic mapping of polygenic scab (Venturia pirina) resistance in an interspecific pear family. Mol Breeding 34, 2179–2189 (2014). https://doi.org/10.1007/s11032-014-0172-6

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  • DOI: https://doi.org/10.1007/s11032-014-0172-6

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