Molecular Breeding

, Volume 34, Issue 1, pp 59-73

First online:

Quantitative trait loci of barley malting quality trait components in the Stellar/01Ab8219 mapping population

  • Emir IslamovicAffiliated withUSDA ARS
  • , Donald E. ObertAffiliated withLimagrain Cereal Seeds Email author 
  • , Allen D. BuddeAffiliated withUSDA ARS Cereal Crop Research Unit
  • , Mark SchmittAffiliated withUSDA ARS Cereal Crop Research Unit
  • , Robert BrunickIIAffiliated withMillerCoors
  • , Andrzej KilianAffiliated withDiversity Arrays Technology
  • , Shiaoman ChaoAffiliated withUSDA ARS
  • , Gerard R. LazoAffiliated withUSDA ARS
  • , Juliet M. MarshallAffiliated withDepartment of Plant, Soil, and Entomological Sciences, University of Idaho
    • , Eric N. JellenAffiliated withDepartment of Plant and Wildlife Sciences, Brigham Young University
    • , Peter J. MaughanAffiliated withDepartment of Plant and Wildlife Sciences, Brigham Young University
    • , Gongshe HuAffiliated withUSDA ARS
    • , Kathy E. KlosAffiliated withUSDA ARS
    • , Ryan H. BrownAffiliated withGeneral Mills, Inc.
    • , Eric W. JacksonAffiliated withGeneral Mills, Inc.

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Malting barley is of high economic and scientific importance. Determining barley grains that are suitable for malting involves measuring malting quality, which is an expensive and complex process. In order to decrease the cost of phenotyping and accelerate the process of developing superior malting barley cultivars, markers for marker-assisted breeding are needed. In this study, we identified quantitative trait loci (QTLs) for malting traits in a Stellar/01Ab8219 F6:8 recombinant inbred line population grown at Aberdeen and Tetonia, Idaho, USA in 2009 and 2010. We identified QTLs associated with malt extract (ME), wort protein, soluble/total protein (S/T), diastatic power (DP), alpha-amylase, beta-glucan (BG) and free amino nitrogen (FAN) at a logarithm of odds score ≥2.5 using a high-density genetic map produced by merging Diversity Arrays Technology markers with the current single nucleotide polymorphism map. Novel QTLs were identified for DP and FAN on chromosome 5H, S/T on 6H, and BG and ME on 7H. Dissection of the genetic regions associated with malting traits suggests the involvement of multiple molecular pathways. The resulting molecular markers may prove useful for barley improvement.


Molecular markers Genetic linkage map QTL mapping Barley Malt Diastatic power Soluble/total protein Free amino nitrogen Amylase