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Quantitative trait locus analysis of Verticillium wilt resistance in an introgressed recombinant inbred population of Upland cotton

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Abstract

Verticillium wilt (VW) of Upland cotton (Gossypium hirsutum L.) is caused by the soil-borne fungal pathogen Verticillium dahlia Kleb. The availability of VW-resistant cultivars is vital for control of this economically important disease, but there is a paucity of Upland cotton breeding lines and cultivars with a high level of resistance to VW. In general, G. barbadense L. (source of Pima cotton) is more VW-resistant than Upland cotton. However, the transfer of VW resistance from G. barbadense to Upland cotton is challenging because of hybrid breakdown in the F2 and successive generations of interspecific populations. We conducted two replicated greenhouse studies (tests 1 and 2) to assess the heritability of VW resistance to a defoliating V. dahliae isolate and identify genetic markers associated with VW resistance in an Upland cotton recombinant inbred mapping population that has stable introgression from Pima cotton. Disease ratings at the seedling stage on several different days after the first inoculation (DAI) in test 1, as well as the percentages of infected and defoliated leaves at 2 DAI in test 2, were found to be low to moderately heritable, indicating the importance of a replicated progeny test in selection for VW resistance. With a newly constructed linkage map consisting of 882 simple sequence repeat, single nucleotide polymorphism, and resistance gene analog–amplified fragment length polymorphism marker loci, we identified a total of 21 quantitative trait loci (QTLs) on 11 chromosomes and two linkage groups associated with VW resistance at several different DAIs in greenhouse tests 1 and 2. The markers associated with the VW resistance QTLs will facilitate fine mapping and cloning of VW resistance genes and genomics-assisted breeding for VW-resistant cultivars.

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Acknowledgments

The research was supported by the USDA-ARS, Cotton Incorporated, and New Mexico Agricultural Experiment Station. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Department of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM, 88003, USA

    Hui Fang, Huiping Zhou & Jinfa Zhang

  2. Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces, NM, 88003, USA

    Soum Sanogo

  3. Institute for Genomic Diversity, Cornell University, Ithaca, NY, 14853, USA

    Alexander E. Lipka

  4. Cotton Fiber Bioscience Research Unit, Southern Regional Research Center, USDA-ARS, 1100 Robert E. Lee Blvd., New Orleans, LA, 70124, USA

    David D. Fang

  5. Crop Germplasm Research Unit, Southern Plains Agricultural Research Center, USDA-ARS, 2881 F & B Road, College Station, TX, 77845, USA

    Richard G. Percy

  6. Southwest Cotton Ginning Research Laboratory, USDA-ARS, Mesilla Park, NM, 88047, USA

    Sidney E. Hughs

  7. Cotton Incorporated, 6399 Weston Parkway, Cary, NC, 27513, USA

    Don C. Jones

  8. Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, 14853, USA

    Michael A. Gore

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  1. Hui Fang
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  8. Don C. Jones
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  9. Michael A. Gore
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  10. Jinfa Zhang
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Correspondence to Michael A. Gore or Jinfa Zhang.

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Fang, H., Zhou, H., Sanogo, S. et al. Quantitative trait locus analysis of Verticillium wilt resistance in an introgressed recombinant inbred population of Upland cotton. Mol Breeding 33, 709–720 (2014). https://doi.org/10.1007/s11032-013-9987-9

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  • DOI: https://doi.org/10.1007/s11032-013-9987-9

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