Abstract
Despite the interest in growing raspberries (Rubus idaeus) in the southeastern USA, production is limited by the lack of cultivars adapted to the climate. One of these major climate adaptations is the ability to tolerate fluctuating winter temperatures. Perennial plants have adapted to these conditions by having high chilling requirements. Breeding efforts are underway for developing adapted cultivars, but breeding improvements in Rubus is a time-consuming process. In order to expedite the breeding process, molecular breeding tools are being developed. In this work, the cross (Rubus parvifolius × Tulameen) × Qualicum was used for the construction of a genetic linkage map and for quantitative trait locus (QTL) analyses of chilling requirement, prickle density, fruit color, fruit shape, fruit size, and flower color. Chilling requirements were determined by measuring bud break in chilled cuttings; all other traits were scored visually. Seven linkage groups were constructed and compared to an established Rubus map. Four regions were associated with chilling requirement, and were mostly consistent across 3 years of evaluation. For the fruit and flower color traits, significant regions were consistent across 2 years, and either one or two QTL were found. Two QTL linked to prickle density were detected; one coincided with previous studies, while the second QTL was found in linkage group 4 and co-localized with the marker for lack of prickles. This region is proposed to contain gene s for the prickle-free trait.
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Acknowledgments
This paper represents a portion of the dissertation submitted by R. M.-B. in partial fulfillment of the requirements for the Ph.D. in Horticulture at North Carolina State University. We thank the Southern Region Small Fruit Consortium and the United States Department of Agriculture–Cooperative State Research, Education, and Extension Service for partial support of the research. We thank the North Carolina Department of Agriculture–Specialty Crop grant program for their funding support. We would like to acknowledge James Ballington for generating the mapping population and the use of germplasm from his breeding program, and Jessica Spencer for helping in the chilling requirement estimations. We would also like to show our appreciation to Emily Buck and Sue Gardiner for the prescreening of the Rubus SSR markers, and the HortResearch Travel Award at Plant and Food Research in Palmerston North, New Zealand.
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Molina-Bravo, R., Fernandez, G.E. & Sosinski, B.R. Quantitative trait locus analysis of tolerance to temperature fluctuations in winter, fruit characteristics, flower color, and prickle-free canes in raspberry. Mol Breeding 33, 267–280 (2014). https://doi.org/10.1007/s11032-013-9947-4
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DOI: https://doi.org/10.1007/s11032-013-9947-4