Skip to main content
SpringerLink
Log in

Physical localization of a novel blue-grained gene derived from Thinopyrum bessarabicum

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Blue wheat grain contains different groups of pigments that can be used for making specialty foods or as food colorants. Thinopyrum bessarabicum, a wild relative of wheat, carries a blue-grained gene on chromosome 4J. In this study, we analyzed the mitotic chromosomes of 159 F7 lines derived from the cross between Triticum aestivum cv. Chinese Spring (CS) and a CS–Th. bessarabicum amphiploid by using multi-color fluorescence in situ hybridization, genomic in situ hybridization, and newly developed chromosome 4J-specific DNA markers. Intact chromosome 4J and various 4J chromosomal segments were identified in the 159 lines. The blue-grained gene of Th. bessarabicum was physically localized to the region between the centromere and FL0.52 on chromosome arm 4JL. The chromosomal location of this gene differed from the location of previously reported blue-grained genes. In addition, a strong dosage effect was observed with this gene. These results suggest that the blue-grained gene in Th. bessarabicum represents a novel gene locus for blue aleurone, designated BaThb. The wheat lines and 4J chromosome-specific molecular markers developed in this study will facilitate the introgression and utilization of BaThb for wheat nutritional quality improvement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abdel-Aal EM, Pierre H (2003) Composition and stability of anthocyanins in blue-grained wheat. J Agric Food Chem 51:2174–2180

    Article  CAS  Google Scholar 

  • Abdel-Aal EM, Young JC, Rabalski I (2006) Anthocyanin composition in black, blue, pink, purple, and red cereal grains. J Agric Food Chem 54:4696–4704

    Article  CAS  Google Scholar 

  • Bedbrook JR, Jones J, O’Dell M, Thompson RD, Flavell RB (1980) A molecular description of telomeric heterochromatin in Secale species. Cell 19:545–560

    Article  PubMed  CAS  Google Scholar 

  • Chen HF, Qian BL, Zhuang LF, Chen QZ, Feng YG, Pei ZY, Qi ZJ, Chen PD, Liu DJ (2007) Molecular marker analysis on common wheat landrace Chinese Spring alien chromosome lines derived from Thinopyrum bessarabicum Löve. Acta Agron Sin 33:1232–1239 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Dawadondup (2009) Development and molecular characterization of wheat–Thinopyrum bessarabicum Löve alien chromosome lines involving 4J and 5J. Master’s thesis. Nanjing Agricultural University, Nanjing

    Google Scholar 

  • Dubcovsky J, Luo MC, Zhong GY, Bransteitter R, Desai A, Kilian A, Kleinhofs A, Dvorak J (1996) Genetic map of diploid wheat, Triticum monococcum L., and its comparison with maps of Hordeum vulgare L. Genetics 143:983–999

    PubMed  CAS  Google Scholar 

  • Gorham J, McDonnell E, Budrewicz E, Wynn Jones RG (1985) Salt tolerance in the Triticeae: growth and solute accumulation in leaves of Thinopyrum bessarabicum. J Exp Bot 36:1021–1031

    Article  CAS  Google Scholar 

  • Hanson BD, Mallory-Smith CA, Shafii B, Thill DC, Zemetra RS (2005) Pollen-mediated gene flow from blue aleurone wheat to other wheat cultivars. Crop Sci 45:1610–1617

    Article  CAS  Google Scholar 

  • Jan CC, Dvorak J, Qualset CO, Soliman KM (1981) Selection and identification of a spontaneous alien chromosome translocation in wheat. Genetics 98:389–398

    PubMed  CAS  Google Scholar 

  • Jiang J, Gill BS (1993) Sequential chromosome banding and in situ hybridization analysis. Genome 36:792–795

    Article  PubMed  CAS  Google Scholar 

  • Keppenne VD, Baenziger S (1990) Inheritance of the blue aleurone trait in diverse wheat crosses. Genome 33:525–529

    Article  Google Scholar 

  • King IP, Forster BP, Law CC, Cant KA, Orford SE, Gorham J, Reader S, Miller TE (1997) Introgression of salt-tolerance genes from Thinopyrum bessarabicum into wheat. New Phytol 137:75–81

    Article  Google Scholar 

  • Ma ZQ, Sorrells ME (1995) Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphisms. Crop Sci 35:1137–1143

    Article  CAS  Google Scholar 

  • McIntyre CL, Pereira S, Moran LB, Apples R (1990) New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33:317–323

    Article  Google Scholar 

  • Metzger RJ, Sebesta E (2004) Registration of three blue-seeded wheat genetic stocks exhibiting xenia. Crop Sci 44:2281–2282

    Article  Google Scholar 

  • Miftahudin RK, Ma X-F, Mahmoud AA, Layton J, Rodriguez Milla MA, Chikmawati T, Ramalingam J, Feril O, Pathan MS, Surlan Momirovic G, Kim S, Chema K, Fang P, Haule L, Struxness H, Birkes J, Yaghoubian C, Skinner R, McAllister J, Nguyen V, Qi LL, Echalier B, Gill BS, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dvořák J, Dilbirligi M, Gill KS, Peng JH, Lapitan NLV, Bermudez-Kandianis SorrellsME, Hossain KG, Kalavacharla V, Kianian SF, Lazo GR, Chao S, Anderson OD, Gonzalez-Hernandez J, Conley EJ, Anderson JA, Choi D-W, Fenton RD, Close TJ, McGuire PE, Qualset CO, Nguyen HT, Gustafson JP (2004) Analysis of expressed sequence tag loci on wheat chromosome group 4. Genetics 168:651–663

    Article  PubMed  CAS  Google Scholar 

  • Morrison LA, Metzger RJ, Lukaszewski AJ (2004) Origin of the blue-aleurone gene in ‘Sebesta Blue’ wheat genetic stocks and a protocol for its use in apomixis screening. Crop Sci 44:2063–2067

    Article  Google Scholar 

  • Mukai Y, Nakahara Y, Yamamoto M (1993) Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36:489–494

    Article  PubMed  CAS  Google Scholar 

  • Niu ZX, Klindworth DL, Friesen TL, Chao SM, Jin Y, Cai XW, Xu SS (2011) Targeted introgression of a wheat stem rust resistance gene by DNA marker-assisted chromosome engineering. Genetics 187:1011–1021

    Article  PubMed  CAS  Google Scholar 

  • Qi ZJ, Du P, Qian BL,·Zhuang LF,·Chen HF, Chen TT, Shen J, Guo J, Feng YG, Pei ZY (2010) Characterization of a wheat–Thinopyrum bessarabicum (T2JS-2BS·2BL) translocation line. Theor Appl Genet 121:589–597

    Google Scholar 

  • Rayburn AL, Gill BS (1986) Molecular identification of the D-genome chromosomes of wheat. J Hered 77:253–255

    CAS  Google Scholar 

  • Shen J (2011) Development and molecular cytogenetic characterization of wheat–Thinopyrum bessarabicum alien chromosome lines. Master’s thesis. Nanjing Agricultural University, Nanjing

    Google Scholar 

  • Singh K, Ghai M, Garg M, Chhuneja P, Kaur P, Schnurbusch T, Keller B, Dhaliwal HS (2007) An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum x T. monococcum RIL population. Theor Appl Genet 115:301–312

    Article  PubMed  CAS  Google Scholar 

  • Tan LJ (2011) Development and identification of structural variations involving chromosomes 1J and 5J of Thinopyrum bessarabicum Löve, Master’s thesis. Nanjing Agricultural University, Nanjing

    Google Scholar 

  • Wang RRC, Larson SR, Jensen KB (2010) Analyses of Thinopyrum bessarabicum, Th. elongatum and Th. junceum chromosomes using EST-SSR markers. Genome 53:1083–1089

    Article  PubMed  CAS  Google Scholar 

  • William MDHM, Mujeeb-Kazi A (1993) Thinopyrum bessarabicum: biochemical and cytological markers for the detection of genetic introgression in its hybrid derivatives with Triticum aestivum L. Theor Appl Genet 86:365–370

    Article  Google Scholar 

  • William MDHM, Mujeeb-Kazi A (1995) Biochemical and molecular diagnostics of Thinopyrum bessarabicum chromosomes in Triticum aestivum germplasm. Theor Appl Genet 90:952–956

    Article  CAS  Google Scholar 

  • Zeven AC (1991) Wheats with purple and blue grains: a review. Euphytica 56:243–258

    Article  Google Scholar 

  • Zhang JY, Li XM, Wang RRC, Cortes A, Rosas V, Mujeebi-Kazi A (2002) Molecular cytogenetic characterization of Eb-genome chromosomes in Thinopyrum bessarabicum disomic addition lines of bread wheat. Plant Sci 163:167–174

    Article  CAS  Google Scholar 

  • Zheng Q, Li B, Mu SM, Zhou HP, Li ZS (2006) Physical mapping of the blue-grained gene(s) from Thinopyrum ponticum by GISH and FISH in a set of translocation lines with different seed colors in wheat. Genome 49:1109–1114

    Article  PubMed  CAS  Google Scholar 

  • Zheng Q, Li B, Li HW, Li ZS (2009) Utilization of blue-grained character in wheat breeding derived from Thinopyrum poticum. J Genet Genomics 36:575–580

    Article  PubMed  Google Scholar 

  • Zhuang LF, Qi ZJ, Ying J, Chen PD, Liu DJ (2003) Development and identification of a whole set of Triticum aestivum–Thinopyrum bessarabicum disomic alien addition lines. Acta Genet Sin 30:919–925 (in Chinese with English abstract)

    PubMed  CAS  Google Scholar 

  • Zhuang LF, Qi ZJ, Chen PD, Feng YG, Liu DJ (2004) Development and identification of Triticum aestivum L.–Thinopyrum bessarabicum Löve chromosome translocations. Agric Sci China 3:649–655

    Google Scholar 

Download references

Acknowledgments

The authors sincerely thank Dr. Mujeeb-Kazi from CIMMYT, Mexico for providing seeds of the amphiploid of wheat Chinese Spring and Th. bessarabicum, Dr. Gill from the Plant Pathology Department, Kansas State University, Manhattan, KS, USA, for providing clones of pSc119.2 and pAs1, and Dr. Xiwen Cai from the Department of Plant Sciences, North Dakota State University for critical review. This project was supported by the National Natural Science Foundation of China (No. 31170302) and the National 863 Program of China (2012AA101105).

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Yuefeng Shen, Jian Shen,  Dawadondup, Lifang Zhuang, Yanzhi Wang, Jing Pu, Yigao Feng, Xiue Wang & Zengjun Qi

  2. Institute of Agriculture, Tibet Academy of Agricultural and Animal Science, Lhasa, 850032, China

    Dawadondup

  3. Heartland Plant Innovations Inc., 217 Southwind Place, Manhattan, KS, 66502, USA

    Chenggen Chu

Authors
  1. Yuefeng Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jian Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dawadondup
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lifang Zhuang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yanzhi Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jing Pu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yigao Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Chenggen Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Xiue Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Zengjun Qi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zengjun Qi.

Additional information

Yuefeng Shen and Jian Shen equally contributed to this manuscript.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 31 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shen, Y., Shen, J., Dawadondup et al. Physical localization of a novel blue-grained gene derived from Thinopyrum bessarabicum . Mol Breeding 31, 195–204 (2013). https://doi.org/10.1007/s11032-012-9783-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-012-9783-y

Keywords

Search

Navigation