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Molecular detection of a gene effective against powdery mildew in the wheat cultivar Liangxing 66

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Abstract

Since it was commercialized in 2008, Liangxing 66 is one of the most widely grown cultivars of wheat (Triticum aestivum L.) in winter and facultative wheat-producing regions in northern China. This cultivar displays broad-spectrum resistance to isolates of powdery mildew. To identify the powdery mildew resistance gene in Liangxing 66, genetic analysis and molecular mapping were conducted using the F2 populations and F2:3 families derived from the reciprocal crosses of Liangxing 66 and the susceptible cultivar Jingshuang 16. A single dominant gene, tentatively designated PmLX66, conferred resistance in Liangxing 66 to the powdery mildew isolate E09. The results of molecular mapping indicated that this gene was located on the short arm of chromosome 5D and flanked by SCAR203 and Xcfd81 at genetic distances of 0.4 and 2.8 cM, respectively, which is similar to the position of locus Pm2. However, PmLX66 and Pm2 showed different reactions to five of the 42 isolates of powdery mildew tested. Together, these results indicated that PmLX66 was most likely an allele of Pm2. Based on its superior yield and agronomic performance, in combination with powdery mildew resistance, Liangxing 66 is useful as a promising parent for control of powdery mildew and for the development of new disease-resistant cultivars.

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Acknowledgments

The authors are grateful to Dr. Y. L. Zhou for providing the powdery mildew isolates E03, E05, E09, E11, E16, E18, E20, E21, and E22. This study was financially supported by the Core Research Budget of Nonprofit Governmental Research Institution (20105), the earmarked fund for Modern Agro-industry Technology Research System (CARS-3-1), the National Program for Developing New Transgenic Varieties in China (2009ZX08002-006B), and the National High-Tech Research and Development Program of China (2011AA100103).

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Authors and Affiliations

  1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China

    Jiang Huang, Hongxing Xu & Diaoguo An

  2. The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Jiang Huang, Zihui Zhao, Fengjing Song, Xiaoming Wang, Yan Huang & Hongjie Li

  3. College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, China

    Zihui Zhao & Yan Huang

  4. Graduated School, Chinese Academy of Sciences, Beijing, 100049, China

    Jiang Huang

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  1. Jiang Huang
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  2. Zihui Zhao
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  3. Fengjing Song
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  4. Xiaoming Wang
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  5. Hongxing Xu
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  6. Yan Huang
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  7. Diaoguo An
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  8. Hongjie Li
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Correspondence to Diaoguo An or Hongjie Li.

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Huang, J., Zhao, Z., Song, F. et al. Molecular detection of a gene effective against powdery mildew in the wheat cultivar Liangxing 66. Mol Breeding 30, 1737–1745 (2012). https://doi.org/10.1007/s11032-012-9757-0

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  • DOI: https://doi.org/10.1007/s11032-012-9757-0

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