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Identification and fine mapping of a major R gene to Magnaporthe oryzae in a broad-spectrum resistant germplasm in rice

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Abstract

Identification of R genes and development of associated molecular markers will facilitate their application in the development of crop cultivars resistant to disease. We evaluated the resistance of a resistant germplasm ‘D69’, 10 monogenic lines, and model cultivar ‘Nipponbare’ to 56 M. oryzae isolates of blast disease in rice. The results demonstrated that only D69 exhibited full-spectrum resistance among the 12 investigated materials. Resistance inheritance in D69 was analyzed using a stable isolate GD08T13 with strong pathogenicity, collected from diseased panicles. A single dominant R gene was revealed and designated as Pi51(t). Through linkage analysis and the development of new markers, Pi51(t) was subsequently delimited to an interval of ~100.8 kb flanked by markers Ind306 and RM19818, where Pi2, Pi9, Piz, Piz-t, Pigm(t), and Pi40(t) reside. Different genotypes identified by linked markers pB8, Pi9-2, zt56591, and T845, and different pathotypes to the same set of isolates, distinguished Pi51(t) from Pi2, Pi9, Piz, and Piz-t. The origin of Pi40(t) in wild rice suggests that Pi51(t) and Pi40(t) are different. Comparison of resistance spectra suggests multiple R genes in D69, making its resistance durable and valuable in breeding programs. The results of this work will facilitate future studies on cloning and functional analysis of blast resistance genes for rice improvement.

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Acknowledgments

We appreciate Dr. Wengui Yan for his critical reading of the manuscript. We are grateful to the International Rice Research Institute and the Institute of Plant Protection, Chinese Academy of Agricultural Sciences for providing the monogenic lines. This research was supported by China Postdoctoral Science Foundation (20110490939), the Scientific and Technological Project of Guangdong Province, China (2011A020102004), the Natural Science Foundation of Guangdong Province, China (S2011020001513), and the earmarked fund for Modern Agro-industry Technology Research System.

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Authors and Affiliations

  1. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Wuming Xiao & Jun Duan

  2. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Qiyun Yang & Xiaoyuan Zhu

  3. National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China

    Wuming Xiao, Hui Wang, Tao Guo, Yongzhu Liu & Zhiqiang Chen

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  1. Wuming Xiao
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  2. Qiyun Yang
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  4. Jun Duan
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  5. Tao Guo
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  6. Yongzhu Liu
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  7. Xiaoyuan Zhu
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  8. Zhiqiang Chen
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Correspondence to Zhiqiang Chen.

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Wuming Xiao and Qiyun Yang contributed equally to this work.

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Xiao, W., Yang, Q., Wang, H. et al. Identification and fine mapping of a major R gene to Magnaporthe oryzae in a broad-spectrum resistant germplasm in rice. Mol Breeding 30, 1715–1726 (2012). https://doi.org/10.1007/s11032-012-9755-2

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