Molecular Breeding

, Volume 29, Issue 2, pp 477–488

Single nucleotide polymorphism in wheat chromosome region harboring Fhb1 for Fusarium head blight resistance

  • Amy N. Bernardo
  • Hongxiang Ma
  • Dadong Zhang
  • Guihua Bai
Article

DOI: 10.1007/s11032-011-9565-y

Cite this article as:
Bernardo, A.N., Ma, H., Zhang, D. et al. Mol Breeding (2012) 29: 477. doi:10.1007/s11032-011-9565-y

Abstract

Fusarium head blight (FHB) is a destructive disease that reduces wheat grain yield and quality. To date, the quantitative trait locus on 3BS (Fhb1) from Sumai 3 has shown the largest effect on FHB resistance. Single nucleotide polymorphism (SNP) is the most common form of genetic variation and is suitable for high-throughput marker-assisted selection (MAS). We analyzed SNPs derived from 23 wheat expressed sequence tags (ESTs) that previously mapped near Fhb1 on chromosome 3BS. Using 71 Ning 7840/Clark BC7F7 recombinant inbred lines and the single-base extension method, we mapped seven SNP markers between Xgwm533 and Xgwm493, flanking markers for Fhb1. Five of the SNPs explained 45–54% of the phenotypic variation for FHB resistance. Haplotype analysis of 63 wheat accessions from eight countries based on SNPs in EST sequences, simple sequence repeats, and sequence tagged sites in the Fhb1 region identified four major groups: (1) US-Clark, (2) Asian, (3) US-Ernie, and (4) Chinese Spring. The Asian group consisted of Chinese and Japanese accessions that carry Fhb1 and could be differentiated from other groups by marker Xsnp3BS-11. All Sumai 3-related accessions formed a subgroup within the Asian group and could be sorted out by Xsnp3BS-8. The SNP markers identified in this study should be useful for MAS of Fhb1 and fine mapping to facilitate cloning of the Fhb1 resistance gene.

Keywords

Single nucleotide polymorphismFhb1Fusarium graminearumMarker-assisted selectionGenetic diversity

Supplementary material

11032_2011_9565_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 13 kb)
11032_2011_9565_MOESM2_ESM.docx (21 kb)
Supplementary material 2 (DOCX 22 kb)
11032_2011_9565_MOESM3_ESM.pdf (194 kb)
Supplementary material 3 (PDF 193 kb)

Copyright information

© Springer Science+Business Media B.V. (outside the USA) 2011

Authors and Affiliations

  • Amy N. Bernardo
    • 1
  • Hongxiang Ma
    • 2
    • 3
  • Dadong Zhang
    • 3
  • Guihua Bai
    • 3
    • 4
  1. 1.Department of Plant PathologyKansas State UniversityManhattanUSA
  2. 2.Plant Genetics and BiotechnologyJAASNanjingChina
  3. 3.Department of AgronomyKansas State UniversityManhattanUSA
  4. 4.Hard Winter Wheat Genetics Research UnitUSDA-ARSManhattanUSA