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Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace

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Abstract

The Spanish landrace-derived inbred line SBCC97, together with other lines from the Spanish Barley Core Collection, displays high resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei. The objective of this study was to map quantitative trait loci (QTLs) for resistance to powdery mildew in a recombinant inbred line population derived from a cross between SBCC97 and the susceptible cultivar ‘Plaisant’. Phenotypic analysis was performed using four B. graminis isolates, and genetic maps were constructed with mainly simple sequence repeat (SSR) markers, following a sequential genotyping strategy. Two major QTLs with large effects were identified on chromosome 7H, and they accounted for up to 45% of the total phenotypic variance. The alleles for resistance at each QTL were contributed by the Spanish parent SBCC97. One locus was mapped to the short arm of chromosome 7HS, and was flanked by the resistance gene analogue (RGA) marker S9202 and the SSR GBM1060. This corresponded to the same chromosomal region in which a major race-specific resistance gene from Hordeum vulgare ssp. spontaneum, designated as mlt, had been identified previously. The second QTL was linked tightly to marker EBmac0755, and it shared its chromosomal location with the qualitative resistance gene Mlf, which has only been described previously in the wild ancestor H. spontaneum. This is the first report of these two QTLs occurring together in cultivated barley, and it paves the way for their use in barley breeding programs that are designed to transfer resistance alleles into elite cultivars.

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References

  • Ayliffe MA, Collins NC, Ellis JG, Pryor A (2000) The maize rp1 rust resistance gene identifies homologues in barley that have been subjected to diversifying selection. Theor Appl Genet 100:1144–1154

    Article  CAS  Google Scholar 

  • Backes G, Graner B, Foroughi-Wehr G, Fischbeck G, Wenzel G, Jahoor A (1995) Localization of quantitative trait loci (QTL) for agronomic important characters by the use of a RFLP map in barley (Hordeum vulgare L.). Theor Appl Genet 90:294–302

    Article  CAS  Google Scholar 

  • Backes G, Schwarz G, Wenzel G, Jahoor A (1996) Comparison between QTL analyses on powdery mildew resistance in barley based on detached primary leaves and on field data. Plant Breed 115:419–421

    Article  Google Scholar 

  • Backes G, Madsen LH, Jaiser H, Stougaard J, Herz M, Mohler V, Jahoor A (2003) Localization of genes for resistance against Blumeria graminis f.sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp. spontaneum) line. Theor Appl Genet 106:353–362

    CAS  PubMed  Google Scholar 

  • Brown JKM, Jørgensen JH (1991) A catalogue of mildew resistance genes in European barley varieties. In: Jørgensen JH (ed) Integrated control of cereal mildews: virulence patterns and their change. Risø National Laboratory, Roskilde, Denmark, pp 263–286

    Google Scholar 

  • Brueggeman R, Rostoks N, Kurdna D, Kilian A, Han F, Chen J, Druka A, Steffenson B, Kleinhofs A (2002) The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. Proc Natl Acad Sci USA 99:9328–9333

    Article  CAS  PubMed  Google Scholar 

  • Bulgarelli D, Collins NC, Tacconi G, Dellaglio E, Brueggeman R, Kleinhofs A, Stanca AM, Valè G (2004) High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley. Theor Appl Genet 108:1401–1408

    Article  CAS  PubMed  Google Scholar 

  • Collins N, Park R, Spielmeyer W, Ellis J, Pryor AJ (2001) Resistance gene analogs in barley and their relationship to rust resistance genes. Genome 44:375–381

    Article  CAS  PubMed  Google Scholar 

  • Dreiseitl A (2007) Variety resistance of winter barley to powdery mildew in the field in 1976–2005. Czech J Genet Plant Breed 43:87–96

    Google Scholar 

  • FAO (2006) FAO statistical yearbook 2005-6 http://www.fao.org/statistics/yearbook/vol_1_1/pdf/b06.pdf

  • Fischbeck G, Jahoor A (1991) The transfer of genes for mildew resistance from Hordeum spontaneum. In: Jørgensen JH (ed) Integrated control of cereal mildews: virulence patterns and their change. Risø National Laboratory, Roskilde, Denmark, pp 247–255

    Google Scholar 

  • Friedt W, Ordon F (2007) Molecular markers for gene pyramiding and disease resistance breeding in barley. In: Varshney RV, Tuberosa R (eds) Genomics-assisted crop improvement: vol. 2: Genomics application in crops. Springer, Netherlands, pp 81–101

    Chapter  Google Scholar 

  • Gullino ML, Kuijpers LAM (1994) Social and political implications of managing plant diseases with restricted fungicides in Europe. Annu Rev Phytopathol 32:559–579

    Article  CAS  PubMed  Google Scholar 

  • Hayes PM, Castro A, Marquez-Cedillo L, Corey A, Henson C, Jones BL, Kling J, Mather D, Matus I, Rossi C, Sato K (2003) Genetic diversity for quantitative inherited agronomic and malting quality traits. In: von Bothmer R et al (eds) Diversity in barley (Hordeum vulgare). Elsevier, Amsterdam, pp 201–226

    Chapter  Google Scholar 

  • Igartua E, Gracia MP, Lasa JM, Medina B, Molina-Cano JL, Montoya JL, Romagosa I (1998) The Spanish barley core collection. Genet Resour Crop Ev 45:475–481

    Article  Google Scholar 

  • Jensen HP, Christensen E, Jørgensen JH (1992) Powdery mildew resistance genes in 127 Northwest European spring barley varieties. Plant Breed 108:210–228

    Article  Google Scholar 

  • Jørgensen JH (1994) Genetics of powdery mildew resistance in barley. Crit Rev Plant Sci 13:97–119

    Article  Google Scholar 

  • Jørgensen JH, Jensen HP (1997) Powdery mildew resistance in barley landrace material. I. Screening for resistance. Euphytica 97:227–233

    Article  Google Scholar 

  • Kølster P, Munk L, Stølen O, Løhde J (1986) Near-isogenic barley lines with genes for resistance to powdery mildew. Crop Sci 26:903–907

    Article  Google Scholar 

  • Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lasa JM, Igartua E, Ciudad FJ, Codesal P, Garcia EV, Gracia MP, Medina B, Romagosa I, Molina-Cano JL, Montoya JL (2001) Morphological and agronomical diversity patterns in the Spanish barley core collection. Hereditas 135:217–225

    Article  CAS  PubMed  Google Scholar 

  • Leister D, Kurth J, Laurie DA, Yano M, Sasaki T, Devos K, Graner A, Schulze-Lefert P (1998) Rapid re-organization of resistance gene homologs in cereal genomes. Proc Natl Acad Sci USA 95:370–375

    Article  CAS  PubMed  Google Scholar 

  • Limpert E, Müller K (1994) Designation of pathotypes of plant pathogens. J Phytopathol 140:346–358

    Article  Google Scholar 

  • Madsen LH, Collins NC, Rakwalska M, Backes G, Sandal N, Krusell L, Jensen J, Waterman EH, Jahoor A, Ayliffe M, Pryor AJ, Langridge P, Schulze-Lefert P, Stougaard J (2003) Barley disease resistance gene analogs of the NBS-LRR class: identification and mapping. Mol Genet Genomics 269:150–161

    CAS  PubMed  Google Scholar 

  • Marcel TC, Varshney RK, Barbieri M, Jafary H, de Kock MJD, Graner A, Niks RE (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theor Appl Genet 114:487–500

    Article  CAS  PubMed  Google Scholar 

  • Martin GB, Bogdanove AJ, Sessa G (2003) Understanding the functions of plant disease resistance proteins. Annu Rev Plant Biol 54:23–61

    Article  CAS  PubMed  Google Scholar 

  • Panstruga R, Molina-Cano JL, Reinstadler A, Müller J (2005) Molecular characterization of mlo mutants in North American two- and six-rowed malting barley cultivars. Mol Plant Pathol 6:315–320

    Article  CAS  PubMed  Google Scholar 

  • Pflieger S, Lefebvre V, Caranta C, Blattes A, Goffinet B, Palloix A (1999) Disease resistance gene analogs as candidates for QTLs involved in pepper-pathogen interactions. Genome 42:1100–1110

    Article  CAS  PubMed  Google Scholar 

  • Pillen K, Binder A, Kreuzkam B, Ramsay L, Waugh R, Förster J, Léon J (2000) Mapping new EMBL-derived barley microsatellites and their use to differentiate German barley cultivars. Theor Appl Genet 101:652–660

    Article  CAS  Google Scholar 

  • Ramsay L, Macaulay M, Degli-Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005

    CAS  PubMed  Google Scholar 

  • Řepková J, Dreiseitl A, Lízal P, Kyjovská Z, Teturová K, Psotková R, Jahoor A (2006) Identification of resistance genes against powdery mildew in four accessions of Hordeum vulgare ssp. spontaneum. Euphytica 151:23–30

    Article  CAS  Google Scholar 

  • Rossi C, Cuesta-Marcos A, Vales I, Gomez-Pando L, Orjeda G, Wise R, Sato K, Hori K, Capettini F, Vivar H, Chen X, Hayes P (2006) Mapping multiple disease resistance genes using a barley mapping population evaluated in Peru, Mexico, and the USA. Mol Breed 18:355–366

    Article  CAS  Google Scholar 

  • Rostoks N, Mudie S, Cardle L, Russell J, Ramsay L, Booth A, Svensson J, Wanamaker S, Walia H, Rodriguez E, Hedley P, Liu H, Morris J, Close T, Marshall D, Waugh R (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Mol Gen Genomics 274:515–527

    Article  CAS  Google Scholar 

  • SAS Institute (1988) SAS/STATTM user’s guide, release 6.03. Edition. Cary, NC: SAS Institute Inc., 1028 p

  • Satagopan JM, Verbel DA, Venkatraman ES, Offit KE, Begg CB (2002) Two-stage designs for gene-disease association studies. Biometrics 58:163–170

    Article  PubMed  Google Scholar 

  • Satagopan JM, Saunak S, Churchill GA (2007) Sequential quantitative trait locus mapping in experimental crosses. Memorial Sloan-Kettering cancer center. Department of epidemiology and biostatistics, working paper series year 2007, paper 7

  • Schmalenbach I, Körber N, Pillen K (2008) Selecting a set of wild barley introgression lines and verification of QTL effects for resistance to powdery mildew and leaf rust. Theor Appl Genet 117:1093–1106

    Article  PubMed  Google Scholar 

  • Schönfeld M, Ragni A, Fischbeck G, Jahoor A (1996) RFLP mapping of three new loci for resistance genes to powdery mildew (Erysiphe graminis f. sp. hordei) in barley. Theor Appl Genet 93:48–56

    Article  Google Scholar 

  • Schweizer GF, Baumer M, Daniel G, Rugel H, Röder MS (1995) RFLP markers linked to scald (Rhynchosporium secalis) resistance gene Rh2 in barley. Theor Appl Genet 90:920–924

    Article  CAS  Google Scholar 

  • Seah S, Sivasithamparam K, Karakousis A, Lagudah ES (1998) Cloning and characterisation of a family of disease resistance gene analogs from wheat and barley. Theor Appl Genet 97:937–945

    Article  CAS  Google Scholar 

  • Sen S, Satagopan JM, Churchill GA (2005) Quantitative trait locus study design from an information perspective. Genetics 170:447–464

    Article  CAS  PubMed  Google Scholar 

  • Silvar C, Casas AM, Kopahnke D, Habekuß A, Schweizer G, Gracia MP, Lasa JM, Ciudad FJ, Molina-Cano JL, Igartua E, Ordon F (2009) Screening the Spanish barley core collection for disease resistance. Plant Breed. doi:10.1111/j.1439-0523.2009.01700.x

  • Thiel T, Michalek W, Varshney RK, Graner A (2003) Exploiting EST databases for the development and characterization of gene derived SSR-markers in barley (Hordeum vulgare L.). Theor Appl Genet 106:411–422

    CAS  PubMed  Google Scholar 

  • Torp J, Jensen HP, Jørgensen JH (1978) Powdery mildew resistance genes in 106 northwest European spring barley varieties. Royal Veterinary & Agricultural University Copenhagen Yearbook 1978, pp 75–102

  • van Ooijen JW, Voorrips RE (2001) JoinMap® 3.0. software for the calculation of genetic linkage maps. Plant Research International, Wageningen, Netherlands

    Google Scholar 

  • Varshney RK, Marcel TC, Ramsay L, Russell J, Röder MS, Stein N, Waugh R, Langridge P, Niks RE, Graner A (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theor Appl Genet 114:1091–1103

    Article  CAS  PubMed  Google Scholar 

  • von Korff M, Wang H, Léon J, Pillen K (2005) AB-QTL analysis in spring barley. I. Detection of resistance genes against powdery mildew, leaf rust and scald introgressed from wild barley. Theor Appl Genet 111:583–590

    Article  CAS  Google Scholar 

  • Wagner C, Schweizer G, Krämer M, Dehmer-Badani AG, Ordon F, Friedt W (2008) The complex quantitative barley-Rhynchosporium secalis interaction: newly identified QTL may represent already known resistance genes. Theor Appl Genet 118:113–122

    Article  CAS  PubMed  Google Scholar 

  • Wang H, Thomas DC, Peer I, Stram DO (2006) Optimal two-stage genotyping designs for genome-wide association scans. Genet Epidemiol 30:356–358

    Article  PubMed  Google Scholar 

  • Wang S, Basten CJ, Baffney P, Zeng ZB (2007) Windows QTL Cartographer 2.5. Bioinformatics Research Center. N.C. State University, Raleigh, NC

    Google Scholar 

  • Williams KJ (2003) The molecular genetics of disease resistance in barley. Aust J Agric Res 54:1065–1079

    Article  CAS  Google Scholar 

  • Yahiaoui S, Igartua E, Moralejo M, Ramsay L, Molina-Cano JL, Ciudad FJ, Lasa JM, Gracia MP, Casas AM (2008) Patterns of genetic and eco-geographical diversity in Spanish barleys. Theor Appl Genet 116:271–282

    Article  CAS  PubMed  Google Scholar 

  • Zeng Z (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    CAS  PubMed  Google Scholar 

  • Zhang Z, Henderson C, Perfect E, Carver TLW, Thomas BJ, Skamnioti P, Gurr SJ (2005) Of genes and genomes, needles and haystacks: Blumeria graminis and functionality. Mol Plant Pathol 6:561–575

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Spanish Ministry of Education and Research (Project AGL2007-63625), and by the European Regional Development Fund. C.S. holds an I3P contract from CSIC. H.D. was supported by a Masters fellowship from IAMZ-CIHEAM.

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Authors and Affiliations

  1. Department of Genetics and Plant Production, Aula Dei Experimental Station, CSIC, P.O. Box 13034, 50080, Zaragoza, Spain

    Cristina Silvar, Hichem Dhif, Ernesto Igartua, Maria P. Gracia, José M. Lasa & Ana M. Casas

  2. Institute for Resistance Research and Stress Tolerance, Julius Kühn-Institute, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Doris Kopahnke & Frank Ordon

Authors
  1. Cristina Silvar
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  2. Hichem Dhif
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  3. Ernesto Igartua
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  4. Doris Kopahnke
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  5. Maria P. Gracia
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  6. José M. Lasa
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  7. Frank Ordon
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  8. Ana M. Casas
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Correspondence to Ernesto Igartua.

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Silvar, C., Dhif, H., Igartua, E. et al. Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace. Mol Breeding 25, 581–592 (2010). https://doi.org/10.1007/s11032-009-9354-z

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  • DOI: https://doi.org/10.1007/s11032-009-9354-z

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