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Single nucleotide polymorphism (SNP) genotyping as basis for developing a PCR-based marker highly diagnostic for potato varieties with high resistance to Globodera pallida pathotype Pa2/3

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Abstract

Globodera pallida is a parasitic root cyst nematode of potato, which causes reduction of crop yield and quality in infested fields. Field populations of G. pallida containing mixtures of pathotypes Pa2 and Pa3 (Pa2/3) are currently most relevant for potato cultivation in middle Europe. Genes for resistance to G. pallida have been introgressed into the cultivated potato gene pool from the wild, tuber bearing Solanum species S. spegazzinii and S. vernei. Selection of resistant genotypes in breeding programs is hampered by the fact that the phenotypic evaluation of resistance to G. pallida is time consuming, costly and often ambiguous. DNA-based markers diagnostic for resistance to G. pallida would facilitate the development of resistant varieties. A tetraploid F1 hybrid family SR-Gpa segregating for quantitative resistance to G.␣pallida was developed and evaluated for resistance to G. pallida population ‘Chavornay’. Two subpopulations of 30 highly resistant and 30 susceptible individuals were selected and genotyped for 96 single nucleotide polymorphism (SNP) markers tagging 12 genomic regions on 10 potato chromosomes. Seven SNPs were found significantly linked to the nematode resistance, which were all located within a resistance ‘hotspot’ on potato chromosome V. A haplotype model for these seven SNPs was deduced from the SNP patterns observed in the SR-Gpa family. A PCR assay ‘HC’ was developed, which specifically detected the SNP haplotype c that was linked with high levels of nematode resistance. The HC marker was only found in accessions of S.␣vernei. Screening with the HC marker 34 potato varieties resistant to G. pallida pathotypes Pa2 and/or Pa3 and 22 susceptible varieties demonstrated that the HC marker was highly diagnostic for presence of high levels of resistance to G. pallida pathotype Pa2/Pa3.

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Acknowledgements

The authors gratefully acknowledge the funding of this work under the GABI program (Genome analysis in the biological system of plants) by BMBF (Bundesministerium für Bildung und Forschung), Project No 0312290 CONQUEST (Genes CONtrolling QUantitativE traits of Solanum Tuberosum). Part of this work was carried out in the department of plant breeding research and yield physiology, headed by Francesco Salamini, and in the department of plant breeding research and genetics, headed by Maarten Koornneef.

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Authors and Affiliations

  1. Max-Planck Institut für Züchtungsforschung, Carl von Linné Weg 10, 50829, Köln, Germany

    Amirali Sattarzadeh, Ute Achenbach, Tamara Rothsteyn & Christiane Gebhardt

  2. SaKa-Ragis Pflanzenzucht GbR, 24340, Windeby, Germany

    Jens Lübeck & Josef Strahwald

  3. Bioplant GmbH, 29574, Ebstorf, Germany

    Eckhard Tacke

  4. Böhm-Nordkartoffel Agrarproduktion GbR, 21337, Lüneburg, Germany

    Hans-Reinhard Hofferbert

Authors
  1. Amirali Sattarzadeh
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  2. Ute Achenbach
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  3. Jens Lübeck
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  4. Josef Strahwald
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  5. Eckhard Tacke
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  6. Hans-Reinhard Hofferbert
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  7. Tamara Rothsteyn
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  8. Christiane Gebhardt
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Correspondence to Christiane Gebhardt.

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Amirali Sattarzadeh and Ute Achenbach contributed equally to the work

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Sattarzadeh, A., Achenbach, U., Lübeck, J. et al. Single nucleotide polymorphism (SNP) genotyping as basis for developing a PCR-based marker highly diagnostic for potato varieties with high resistance to Globodera pallida pathotype Pa2/3. Mol Breeding 18, 301–312 (2006). https://doi.org/10.1007/s11032-006-9026-1

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  • DOI: https://doi.org/10.1007/s11032-006-9026-1

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