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Trans-cinnamaldehyde protected PC12 cells against oxygen and glucose deprivation/reperfusion (OGD/R)-induced injury via anti-apoptosis and anti-oxidative stress

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Abstract

Ischemia stroke is the major cause of mortality and permanent neurological disability with little definitive therapeutic options. This cerebral ischemic injury leads to the oxidative stress and eventually cell death. We hypothesized that treatment of this condition with the trans-cinnamaldehyde(TC) could protect cells from ischemic and reperfusion injury. Oxygen and glucose deprivation/reperfusion (OGD/R) was used as an in vitro model of hypoxic ischemic injury in present study. MTT was used to evaluate the protective effects of TC. Next, we tested whether TC reduced the production of reactive oxygen species (ROS). Besides, experiments were performed to determine whether or not the mitochondrial membrane potential was affected. Furthermore, the inhibiters of NO and PI3 K were used to determine the initial mechanisms. TC treatment improved cell viability, reduced intracellular ROS, and increased MMP. Further, the inhibition of NO or PI3 K significantly reduced TC’s protective effects. These findings suggest that TC might be a promising agent for ischemic stroke.

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Acknowledgments

This study was supported by the National Science and Technology Key Program: The research on the material basis and modern preparations in the treatment of stroke with Hui Ethnic Herbal Fragrant Remedy (2013BAI11B07) and Ningxia Hui Autonomous Region Science and Technology Support Program: The novel drug development for Hui Ethnic Herbal Specific Remedy (2015BAK45B01) (2012 [17]).

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    Authors and Affiliations

    1. Department of Pharmacology, Ningxia Medical University, Ningxia Hui Autonomous Region, 1160 Shengli Street, Yinchuan, 750004, People’s Republic of China

      Xue Qi, Ru Zhou, Yue Liu, Jing Wang, Wan-Nian Zhang, Yu-Xiang Li & Jian-Qiang Yu

    2. Department of Pharmacology, Peking University, Health Science Center, Beijing, 100191, People’s Republic of China

      Huan-Ran Tan

    3. Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Ningxia Hui Autonomous Region, Yinchuan, 750004, People’s Republic of China

      Yang Niu

    4. Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, People’s Republic of China

      Tao Sun

    5. College of Nursing, Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, People’s Republic of China

      Yu-Xiang Li

    6. Ningxia Hui Medicine Modern Engineering Research Center and Collaborative Innovation Center, Ningxia Medical University, Ningxia Hui Autonomous Region, Yinchuan, 750004, People’s Republic of China

      Jian-Qiang Yu

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    1. Xue Qi
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    Xue Qi and Ru Zhou have contributed equally to this work.

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    Qi, X., Zhou, R., Liu, Y. et al. Trans-cinnamaldehyde protected PC12 cells against oxygen and glucose deprivation/reperfusion (OGD/R)-induced injury via anti-apoptosis and anti-oxidative stress. Mol Cell Biochem 421, 67–74 (2016). https://doi.org/10.1007/s11010-016-2785-z

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