Molecular and Cellular Biochemistry

, Volume 411, Issue 1, pp 11–21

Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1

  • Bhakti R. Pathak
  • Ananya A. Breed
  • Snehal Apte
  • Kshitish Acharya
  • Smita D. Mahale
Article

DOI: 10.1007/s11010-015-2564-2

Cite this article as:
Pathak, B.R., Breed, A.A., Apte, S. et al. Mol Cell Biochem (2016) 411: 11. doi:10.1007/s11010-015-2564-2

Abstract

Cysteine-rich secretory protein 3 (CRISP-3) is upregulated in prostate cancer as compared to the normal prostate tissue. Higher expression of CRISP-3 has been linked to poor prognosis and hence it has been thought to act as a prognostic marker for prostate cancer. It is proposed to have a role in innate immunity but its role in prostate cancer is still unknown. In order to understand its function, its expression was stably knocked down in LNCaP cells. CRISP-3 knockdown did not affect cell viability but resulted in reduced invasiveness. Global gene expression changes upon CRISP-3 knockdown were identified by microarray analysis. Microarray data were quantitatively validated by evaluating the expression of seven candidate genes in three independent stable clones. Functional annotation of the differentially expressed genes identified cell adhesion, cell motility, and ion transport to be affected among other biological processes. Prostate-specific antigen (PSA, also known as Kallikrein 3) was the top most downregulated gene whose expression was also validated at protein level. Interestingly, expression of Annexin A1 (ANXA1), a known anti-inflammatory protein, was upregulated upon CRISP-3 knockdown. Re-introduction of CRISP-3 into the knockdown clone reversed the effect on invasiveness and also led to increased PSA expression. These results suggest that overexpression of CRISP-3 in prostate tumor may maintain higher PSA expression and lower ANXA1 expression. Our data also indicate that poor prognosis associated with higher CRISP-3 expression could be due to its role in cell invasion.

Keywords

CRISP-3 Prostate cancer Prognosis shRNA PSA ANXA1 

Supplementary material

11010_2015_2564_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 kb)
11010_2015_2564_MOESM2_ESM.doc (38 kb)
Supplementary material 2 (DOC 38 kb)

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Bhakti R. Pathak
    • 1
  • Ananya A. Breed
    • 1
  • Snehal Apte
    • 1
  • Kshitish Acharya
    • 2
    • 3
  • Smita D. Mahale
    • 1
  1. 1.Division of Structural BiologyNational Institute for Research in Reproductive Health, Indian Council of Medical ResearchMumbaiIndia
  2. 2.Institute of Bioinformatics and Applied Biotechnology (IBAB)BengaluruIndia
  3. 3.Shodhaka Life Sciences, Pvt. Ltd.IBABBengaluruIndia